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PDBsum entry 4mn8
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Transferase/transferase receptor
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PDB id
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4mn8
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Contents |
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759 a.a.
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175 a.a.
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21 a.a.
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References listed in PDB file
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Key reference
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Title
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Structural basis for flg22-Induced activation of the arabidopsis fls2-Bak1 immune complex.
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Authors
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Y.Sun,
L.Li,
A.P.Macho,
Z.Han,
Z.Hu,
C.Zipfel,
J.M.Zhou,
J.Chai.
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Ref.
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Science, 2013,
342,
624-628.
[DOI no: ]
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PubMed id
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Abstract
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Flagellin perception in Arabidopsis is through recognition of its highly
conserved N-terminal epitope (flg22) by flagellin-sensitive 2 (FLS2). Flg22
binding induces FLS2 heteromerization with BRASSINOSTEROID INSENSITIVE
1-associated kinase 1 (BAK1) and their reciprocal activation followed by plant
immunity. Here, we report the crystal structure of FLS2 and BAK1 ectodomains
complexed with flg22 at 3.06 angstroms. A conserved and a nonconserved site from
the inner surface of the FLS2 solenoid recognize the C- and N-terminal segment
of flg22, respectively, without oligomerization or conformational changes in the
FLS2 ectodomain. Besides directly interacting with FLS2, BAK1 acts as a
co-receptor by recognizing the C terminus of the FLS2-bound flg22. Our data
reveal the molecular mechanisms underlying FLS2-BAK1 complex recognition of
flg22 and provide insight into the immune receptor complex activation.
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