PDBsum entry 4lvr

Transport protein

PDB id: 4lvr

Contents

Protein chain

120 a.a.

PDB id:

4lvr

Name:

Transport protein

Title:

Crystal structure of ift81 n-terminal domain

Structure:

Intraflagellar transport protein 81. Chain: a. Fragment: n-terminal domain (unp residues 1-124). Synonym: ift81. Engineered: yes

Source:

Chlamydomonas reinhardtii. Organism_taxid: 3055. Gene: ift81, chlredraft_138649. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.60Å R-factor: 0.255 R-free: 0.285

Authors:

S.Bhogaraju

Key ref:

S.Bhogaraju et al. (2013). Molecular basis of tubulin transport within the cilium by IFT74 and IFT81. Science, 341, 1009-1012. PubMed id: 23990561 DOI: 10.1126/science.1240985

Date:

26-Jul-13 Release date: 11-Sep-13

Protein chain

Q68RJ5  (IFT81_CHLRE) -  Intraflagellar transport protein 81 from Chlamydomonas reinhardtii

Seq: 683 a.a.

Struc: 120 a.a.

Enzyme reactions

• Enzyme class: E.C.?

DOI no: 10.1126/science.1240985

Science 341:1009-1012 (2013)

PubMed id: 23990561

Molecular basis of tubulin transport within the cilium by IFT74 and IFT81.

S.Bhogaraju, L.Cajanek, C.Fort, T.Blisnick, K.Weber, M.Taschner, N.Mizuno, S.Lamla, P.Bastin, E.A.Nigg, E.Lorentzen.

ABSTRACT

Intraflagellar transport (IFT) of ciliary precursors such as tubulin from the cytoplasm to the ciliary tip is involved in the construction of the cilium, a hairlike organelle found on most eukaryotic cells. However, the molecular mechanisms of IFT are poorly understood. Here, we found that the two core IFT proteins IFT74 and IFT81 form a tubulin-binding module and mapped the interaction to a calponin homology domain of IFT81 and a highly basic domain in IFT74. Knockdown of IFT81 and rescue experiments with point mutants showed that tubulin binding by IFT81 was required for ciliogenesis in human cells.