UniProt functional annotation for P33176



	UniProt functional annotation for P33176

UniProt code: P33176.

Organism: Homo sapiens (Human).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.

Function: Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner (By similarity). Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2- dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes (PubMed:20386726). Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity). Through binding with PLEKHM2 and ARL8B, directs lysosome movement toward microtubule plus ends (Probable). Involved in NK cell-mediated cytotoxicity. Drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (PubMed:24088571). {ECO:0000250|UniProtKB:Q2PQA9, ECO:0000250|UniProtKB:Q61768, ECO:0000269|PubMed:20386726, ECO:0000269|PubMed:24088571, ECO:0000305|PubMed:22172677, ECO:0000305|PubMed:24088571}.

Subunit: Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1 and PPP1R42 (By similarity). Interacts with SYBU (PubMed:15459722). Interacts with JAKMIP1 (PubMed:17532644). Interacts with PLEKHM2 (PubMed:15905402). Interacts with ECPAS (PubMed:20682791). Interacts with ZFYVE27 (By similarity). Found in a complex with OGT, RHOT1, RHOT2 and TRAK1 (PubMed:24995978). Interacts with APP (via cytoplasmic domain) (PubMed:23011729). {ECO:0000250|UniProtKB:Q61768, ECO:0000269|PubMed:12368902, ECO:0000269|PubMed:15459722, ECO:0000269|PubMed:15905402, ECO:0000269|PubMed:17532644, ECO:0000269|PubMed:20682791, ECO:0000269|PubMed:23011729, ECO:0000269|PubMed:24995978, ECO:0000269|PubMed:8606779}.

Subcellular location: Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q2PQA9}. Cytolytic granule membrane {ECO:0000269|PubMed:24088571}. Lysosome membrane {ECO:0000269|PubMed:22172677, ECO:0000269|PubMed:24088571}; Peripheral membrane protein {ECO:0000305|PubMed:22172677, ECO:0000305|PubMed:24088571}; Cytoplasmic side {ECO:0000305|PubMed:22172677, ECO:0000305|PubMed:24088571}. Note=Uniformly distributed between soma and neurites in hippocampal neurons. {ECO:0000250|UniProtKB:Q2PQA9}.

Domain: Composed of three structural domains: a large globular N- terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.

Similarity: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily. {ECO:0000255|PROSITE- ProRule:PRU00283}.

Annotations taken from UniProtKB at the EBI.


Organism:		Homo sapiens (Human).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.



Function:		Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner (By similarity). Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2- dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes (PubMed:20386726). Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity). Through binding with PLEKHM2 and ARL8B, directs lysosome movement toward microtubule plus ends (Probable). Involved in NK cell-mediated cytotoxicity. Drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (PubMed:24088571). {ECO:0000250\|UniProtKB:Q2PQA9, ECO:0000250\|UniProtKB:Q61768, ECO:0000269\|PubMed:20386726, ECO:0000269\|PubMed:24088571, ECO:0000305\|PubMed:22172677, ECO:0000305\|PubMed:24088571}.


Subunit:		Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1 and PPP1R42 (By similarity). Interacts with SYBU (PubMed:15459722). Interacts with JAKMIP1 (PubMed:17532644). Interacts with PLEKHM2 (PubMed:15905402). Interacts with ECPAS (PubMed:20682791). Interacts with ZFYVE27 (By similarity). Found in a complex with OGT, RHOT1, RHOT2 and TRAK1 (PubMed:24995978). Interacts with APP (via cytoplasmic domain) (PubMed:23011729). {ECO:0000250\|UniProtKB:Q61768, ECO:0000269\|PubMed:12368902, ECO:0000269\|PubMed:15459722, ECO:0000269\|PubMed:15905402, ECO:0000269\|PubMed:17532644, ECO:0000269\|PubMed:20682791, ECO:0000269\|PubMed:23011729, ECO:0000269\|PubMed:24995978, ECO:0000269\|PubMed:8606779}.
Subcellular location:		Cytoplasm, cytoskeleton {ECO:0000250\|UniProtKB:Q2PQA9}. Cytolytic granule membrane {ECO:0000269\|PubMed:24088571}. Lysosome membrane {ECO:0000269\|PubMed:22172677, ECO:0000269\|PubMed:24088571}; Peripheral membrane protein {ECO:0000305\|PubMed:22172677, ECO:0000305\|PubMed:24088571}; Cytoplasmic side {ECO:0000305\|PubMed:22172677, ECO:0000305\|PubMed:24088571}. Note=Uniformly distributed between soma and neurites in hippocampal neurons. {ECO:0000250\|UniProtKB:Q2PQA9}.
Domain:		Composed of three structural domains: a large globular N- terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
Similarity:		Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily. {ECO:0000255\|PROSITE- ProRule:PRU00283}.