UniProt functional annotation for P70610



	UniProt functional annotation for P70610

UniProt code: P70610.

Organism: Rattus norvegicus (Rat).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus.

Function: Calcium sensor which positively regulates SNARE-dependent fusion of vesicles with membranes. Binds phospholipids in a calcium- dependent manner and may act at the priming stage of fusion by modifying membrane curvature to stimulate fusion. Involved in calcium- triggered exocytosis in chromaffin cells and calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Involved both in glucose-stimulated insulin secretion in pancreatic cells and insulin-dependent GLUT4 transport to the plasma membrane in adipocytes. {ECO:0000269|PubMed:18596155, ECO:0000269|PubMed:20150444}.

Cofactor: Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000255|PROSITE-ProRule:PRU00041};

Subunit: Interacts with STX4; the interaction is calcium-dependent, increased by insulin and glucose, and mediates vesicle fusion with plasma membrane in pancreatic cells and adipocytes. Interacts with STXBP3; the interaction is direct, occurs at the cell membrane and regulates glucose-stimulated insulin secretion (By similarity). Interacts with cytoplasmic dynein light chain DYNLT1. Interacts with the SNARE (soluble N-ethylmaleimide-sensitive factor attached protein receptor) complex composed of SNAP25, STX1A and VAMP2; the interaction is calcium-dependent and competitive with SYT1. May interact with UNC13A; the interaction mediates targeting to the plasma membrane. {ECO:0000250, ECO:0000269|PubMed:15033971, ECO:0000269|PubMed:18596155, ECO:0000269|PubMed:20150444, ECO:0000269|PubMed:9804756}.

Subcellular location: Cytoplasm. Cytoplasmic granule {ECO:0000250}. Cell membrane; Peripheral membrane protein. Note=Translocates to the plasma membrane in a calcium-dependent manner.

Tissue specificity: Expressed in brain; highly enriched in neurons. {ECO:0000269|PubMed:9115738}.

Domain: C2 domain 1 is involved in binding calcium and phospholipids. C2 domain 2 may also play a role in the calcium-dependent targeting to membranes (By similarity). {ECO:0000250}.

Annotations taken from UniProtKB at the EBI.


Organism:		Rattus norvegicus (Rat).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus.



Function:		Calcium sensor which positively regulates SNARE-dependent fusion of vesicles with membranes. Binds phospholipids in a calcium- dependent manner and may act at the priming stage of fusion by modifying membrane curvature to stimulate fusion. Involved in calcium- triggered exocytosis in chromaffin cells and calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Involved both in glucose-stimulated insulin secretion in pancreatic cells and insulin-dependent GLUT4 transport to the plasma membrane in adipocytes. {ECO:0000269\|PubMed:18596155, ECO:0000269\|PubMed:20150444}.


Cofactor:		Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000255\|PROSITE-ProRule:PRU00041};
Subunit:		Interacts with STX4; the interaction is calcium-dependent, increased by insulin and glucose, and mediates vesicle fusion with plasma membrane in pancreatic cells and adipocytes. Interacts with STXBP3; the interaction is direct, occurs at the cell membrane and regulates glucose-stimulated insulin secretion (By similarity). Interacts with cytoplasmic dynein light chain DYNLT1. Interacts with the SNARE (soluble N-ethylmaleimide-sensitive factor attached protein receptor) complex composed of SNAP25, STX1A and VAMP2; the interaction is calcium-dependent and competitive with SYT1. May interact with UNC13A; the interaction mediates targeting to the plasma membrane. {ECO:0000250, ECO:0000269\|PubMed:15033971, ECO:0000269\|PubMed:18596155, ECO:0000269\|PubMed:20150444, ECO:0000269\|PubMed:9804756}.
Subcellular location:		Cytoplasm. Cytoplasmic granule {ECO:0000250}. Cell membrane; Peripheral membrane protein. Note=Translocates to the plasma membrane in a calcium-dependent manner.
Tissue specificity:		Expressed in brain; highly enriched in neurons. {ECO:0000269\|PubMed:9115738}.
Domain:		C2 domain 1 is involved in binding calcium and phospholipids. C2 domain 2 may also play a role in the calcium-dependent targeting to membranes (By similarity). {ECO:0000250}.