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PDBsum entry 4l8r
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RNA/RNA binding protein/hydrolase
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PDB id
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4l8r
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References listed in PDB file
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Key reference
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Title
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Structure of histone mRNA stem-Loop, Human stem-Loop binding protein, And 3'Hexo ternary complex.
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Authors
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D.Tan,
W.F.Marzluff,
Z.Dominski,
L.Tong.
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Ref.
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Science, 2013,
339,
318-321.
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PubMed id
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Abstract
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Metazoan replication-dependent histone messenger RNAs (mRNAs) have a conserved
stem-loop (SL) at their 3'-end. The stem-loop binding protein (SLBP)
specifically recognizes the SL to regulate histone mRNA metabolism, and the
3'-5' exonuclease 3'hExo trims its 3'-end after processing. We report the
crystal structure of a ternary complex of human SLBP RNA binding domain, human
3'hExo, and a 26-nucleotide SL RNA. Only one base of the SL is recognized
specifically by SLBP, and the two proteins primarily recognize the shape of the
RNA. SLBP and 3'hExo have no direct contact with each other, and induced
structural changes in the loop of the SL mediate their cooperative binding. The
3' flanking sequence is positioned in the 3'hExo active site, but the ternary
complex limits the extent of trimming.
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