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PDBsum entry 4l7x
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Cell cycle, gene regulation
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PDB id
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4l7x
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References listed in PDB file
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Key reference
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Title
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Dido3 phd modulates cell differentiation and division.
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Authors
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J.Gatchalian,
A.Fütterer,
S.B.Rothbart,
Q.Tong,
H.Rincon-Arano,
A.Sánchez de diego,
M.Groudine,
B.D.Strahl,
C.Martínez-A,
K.H.Van wely,
T.G.Kutateladze.
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Ref.
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Cell Rep, 2013,
4,
148-158.
[DOI no: ]
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PubMed id
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Abstract
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Death Inducer Obliterator 3 (Dido3) is implicated in the maintenance of stem
cell genomic stability and tumorigenesis. Here, we show that Dido3 regulates the
expression of stemness genes in embryonic stem cells through its plant
homeodomain (PHD) finger. Binding of Dido3 PHD to histone H3K4me3 is disrupted
by threonine phosphorylation that triggers Dido3 translocation from chromatin to
the mitotic spindle. The crystal structure of Dido3 PHD in complex with H3K4me3
reveals an atypical aromatic-cage-like binding site that contains a histidine
residue. Biochemical, structural, and mutational analyses of the binding
mechanism identified the determinants of specificity and affinity and explained
the inability of homologous PHF3 to bind H3K4me3. Together, our findings reveal
a link between the transcriptional control in embryonic development and
regulation of cell division.
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