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UniProt functional annotation for O16299 | ||
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| UniProt code: O16299. |
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| Organism: | |
Caenorhabditis elegans. |
| Taxonomy: | |
Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis. |
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| Function: | |
Has a role in spindle assembly which acts in the progression through mitosis during embryogenesis. Required for fertility. {ECO:0000269|PubMed:17878235}. |
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| Catalytic activity: | |
Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; Evidence={ECO:0000269|PubMed:15581640}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:15581640}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.44 mM for ATP {ECO:0000269|PubMed:15581640}; Vmax=225 nmol/min/mg enzyme {ECO:0000269|PubMed:15581640}; Note=At 25 degrees Celsius and pH 8.0.; pH dependence: Optimum pH is 8.0-8.8. {ECO:0000269|PubMed:15581640}; Temperature dependence: Optimum temperature is 25-30 degrees Celsius. {ECO:0000269|PubMed:15581640}; |
| Subunit: | |
Hexamer. {ECO:0000269|PubMed:16621600}. |
| Subcellular location: | |
Nucleus {ECO:0000269|PubMed:17878235}. |
| Tissue specificity: | |
Expressed in germ cells. {ECO:0000269|PubMed:17878235}. |
| Disruption phenotype: | |
Sterility owing to depletion of germ cells. {ECO:0000269|PubMed:17878235}. |
| Similarity: | |
Belongs to the AAA ATPase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.