PDBsum entry 4kit

RNA binding protein

PDB id: 4kit

Contents

Protein chains

1722 a.a.

269 a.a.

Ligands

ADP ×2

Metals

_MG

References listed in PDB file

Key reference

• Title: Inhibition of RNA helicase brr2 by the c-Terminal tail of the spliceosomal protein prp8.
• Authors: S.Mozaffari-Jovin, T.Wandersleben, K.F.Santos, C.L.Will, R.Lührmann, M.C.Wahl.
• Ref.: Science, 2013, 341, 80-84. [DOI no: 10.1126/science.1237515]
• PubMed id: 23704370

Abstract

The Ski2-like RNA helicase Brr2 is a core component of the spliceosome that must be tightly regulated to ensure correct timing of spliceosome activation. Little is known about mechanisms of regulation of Ski2-like helicases by protein cofactors. Here we show by crystal structure and biochemical analyses that the Prp8 protein, a major regulator of the spliceosome, can insert its C-terminal tail into Brr2's RNA-binding tunnel, thereby intermittently blocking Brr2's RNA-binding, adenosine triphosphatase, and U4/U6 unwinding activities. Inefficient Brr2 repression is the only recognizable phenotype associated with certain retinitis pigmentosa-linked Prp8 mutations that map to its C-terminal tail. Our data show how a Ski2-like RNA helicase can be reversibly inhibited by a protein cofactor that directly competes with RNA substrate binding.