PDBsum entry 4kf7

Structural protein

PDB id: 4kf7

Contents

Protein chain

1096 a.a.

Waters ×128

PDB id:

4kf7

Name:

Structural protein

Title:

Nup188(aa1-1160) from myceliophthora thermophila

Structure:

Nup188. Chain: a. Fragment: n-terminal domain (unp residues 1-1160). Engineered: yes

Source:

Myceliophthora thermophila. Organism_taxid: 78579. Gene: mycth_2303581. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.65Å R-factor: 0.183 R-free: 0.225

Authors:

T.U.Schwartz,K.R.Andersen

Key ref:

K.R.Andersen et al. (2013). Scaffold nucleoporins Nup188 and Nup192 share structural and functional properties with nuclear transport receptors. Elife, 2, e00745. PubMed id: 23795296 DOI: 10.7554/eLife.00745

Date:

26-Apr-13 Release date: 19-Jun-13

Protein chain

G2QD05  (G2QD05_MYCTT) -  Nucleoporin NUP188 from Thermothelomyces thermophilus (strain ATCC 42464 / BCRC 31852 / DSM 1799)

Seq: 1827 a.a.

Struc: 1096 a.a.

DOI no: 10.7554/eLife.00745

Elife 2:e00745 (2013)

PubMed id: 23795296

Scaffold nucleoporins Nup188 and Nup192 share structural and functional properties with nuclear transport receptors.

K.R.Andersen, E.Onischenko, J.H.Tang, P.Kumar, J.Z.Chen, A.Ulrich, J.T.Liphardt, K.Weis, T.U.Schwartz.

ABSTRACT

Nucleocytoplasmic transport is mediated by nuclear pore complexes (NPCs) embedded in the nuclear envelope. About 30 different proteins (nucleoporins, nups) arrange around a central eightfold rotational axis to build the modular NPC. Nup188 and Nup192 are related and evolutionary conserved, large nucleoporins that are part of the NPC scaffold. Here we determine the structure of Nup188. The protein folds into an extended stack of helices where an N-terminal 130 kDa segment forms an intricate closed ring, while the C-terminal region is a more regular, superhelical structure. Overall, the structure has distant similarity with flexible S-shaped nuclear transport receptors (NTRs). Intriguingly, like NTRs, both Nup188 and Nup192 specifically bind FG-repeats and are able to translocate through NPCs by facilitated diffusion. This blurs the existing dogma of a clear distinction between stationary nups and soluble NTRs and suggests an evolutionary relationship between the NPC and the soluble nuclear transport machinery. DOI:http://dx.doi.org/10.7554/eLife.00745.001.