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UniProt functional annotation for Q02899 | ||
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| UniProt code: Q02899. |
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| Organism: | |
Saccharomyces pastorianus (Lager yeast) (Saccharomyces cerevisiae x Saccharomyces eubayanus). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
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| Function: | |
Flavin-dependent enoate reductase that catalyzes the chemo- and stereoslective hydrogenation of electron-poor alkenes. The enzyme is reduced by NADPH, and oxygen, quinones, and alpha,beta- unsaturated aldehydes and ketones can act as electron acceptors to complete catalytic turnover. The physiological oxidant remains elusive. {ECO:0000269|PubMed:3944085, ECO:0000269|PubMed:9830019}. |
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| Catalytic activity: | |
Reaction=A + H(+) + NADPH = AH2 + NADP(+); Xref=Rhea:RHEA:13149, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:17499, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.6.99.1; Evidence={ECO:0000269|PubMed:9830019, ECO:0000305|PubMed:8454584}; |
| Cofactor: | |
Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000269|PubMed:11668181, ECO:0000269|PubMed:7881908, ECO:0000269|PubMed:9830019, ECO:0000305|PubMed:8454584}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=11.4 uM for alpha-NADPH {ECO:0000269|PubMed:3944085}; KM=7.7 uM for beta-NADPH {ECO:0000269|PubMed:3944085}; KM=1.18 mM for oxygen (with alpha-NADPH a substrate) {ECO:0000269|PubMed:3944085}; KM=0.88 mM for oxygen (with beta-NADPH a substrate) {ECO:0000269|PubMed:3944085}; |
| Subunit: | |
Homodimer or heterodimer. {ECO:0000269|PubMed:11668181, ECO:0000269|PubMed:7881908, ECO:0000269|PubMed:9830019}. |
| Mass spectrometry: | |
Mass=44890; Method=Electrospray; Evidence={ECO:0000269|PubMed:7836424, ECO:0000269|PubMed:8454584}; |
| Similarity: | |
Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.