UniProt functional annotation for P62483



	UniProt functional annotation for P62483

UniProt code: P62483.

Organism: Rattus norvegicus (Rat).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus.

Function: Cytoplasmic potassium channel subunit that modulates the characteristics of the channel-forming alpha-subunits (PubMed:9763623, PubMed:21357749). Contributes to the regulation of nerve signaling, and prevents neuronal hyperexcitability (By similarity). Promotes expression of the pore-forming alpha subunits at the cell membrane, and thereby increases channel activity (PubMed:10896669, PubMed:16770729, PubMed:18003609, PubMed:21357749). Promotes potassium channel closure via a mechanism that does not involve physical obstruction of the channel pore (PubMed:21357749). Modulates the functional properties of KCNA4 (PubMed:9763623). Modulates the functional properties of KCNA5 (By similarity). Enhances KCNB2 channel activity (By similarity). Binds NADPH and has NADPH-dependent aldoketoreductase activity (PubMed:18672894, PubMed:21209188). Has broad substrate specificity and can catalyze the reduction of methylglyoxal, 9,10-phenanthrenequinone, prostaglandin J2, 4-nitrobenzaldehyde, 4-nitroacetophenone and 4-oxo- trans-2-nonenal (in vitro) (PubMed:18672894). {ECO:0000250|UniProtKB:P62482, ECO:0000269|PubMed:10896669, ECO:0000269|PubMed:18003609, ECO:0000269|PubMed:18672894, ECO:0000269|PubMed:21209188, ECO:0000269|PubMed:21357749, ECO:0000269|PubMed:9763623}.

Biophysicochemical properties: pH dependence: Optimum pH is 7.2-7.4.;

Subunit: Homotetramer (PubMed:10884227, PubMed:16002581, PubMed:18004376, PubMed:18806782, PubMed:20534430, PubMed:20360102, PubMed:23705070). Interaction with tetrameric potassium channel alpha subunits gives rise to a heterooctamer (PubMed:10884227, PubMed:16002581, PubMed:18004376, PubMed:18806782, PubMed:20534430, PubMed:20360102, PubMed:23705070). Identified in potassium channel complexes containing KCNA1, KCNA2, KCNA4, KCNA5, KCNA6, KCNAB1 and KCNAB2 (PubMed:9334400). Interacts with KCNA1 (PubMed:23318870, PubMed:10884227). Interacts with KCNA2 (PubMed:18003609, PubMed:21357749, PubMed:23318870, PubMed:16002581, PubMed:18004376, PubMed:18806782, PubMed:20534430, PubMed:20360102, PubMed:23705070). Interacts with KCNA4 and KCND3 (PubMed:12860406). Interacts with KCNA5 (By similarity). Interacts with KCNB2 (By similarity). Interacts (in unphosphorylated form) with MAPRE1 (PubMed:21357749). Forms a ternary complex with SQSTM1 and PRKCZ (PubMed:10477520). {ECO:0000250|UniProtKB:P62482, ECO:0000269|PubMed:10477520, ECO:0000269|PubMed:10884227, ECO:0000269|PubMed:12860406, ECO:0000269|PubMed:16002581, ECO:0000269|PubMed:18003609, ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:18806782, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430, ECO:0000269|PubMed:21357749, ECO:0000269|PubMed:23318870, ECO:0000269|PubMed:23705070, ECO:0000269|PubMed:9334400, ECO:0000269|PubMed:9763623}.

Subcellular location: Cytoplasm {ECO:0000269|PubMed:21357749}. Membrane {ECO:0000269|PubMed:21357749, ECO:0000269|PubMed:9334400}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Cell membrane {ECO:0000269|PubMed:23318870}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Cell projection, axon {ECO:0000269|PubMed:21357749, ECO:0000269|PubMed:9334400}. Cell junction, synapse, synaptosome {ECO:0000269|PubMed:21357749}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:21357749}. Note=Recruited to the cytoplasmic side of the cell membrane via its interaction with pore-forming potassium channel alpha subunits. Associates with microtubules when unphosphorylated (PubMed:21357749). {ECO:0000269|PubMed:21357749, ECO:0000305}.

Tissue specificity: Detected in brain (PubMed:9334400, PubMed:21357749). Detected in the middle third of the molecular layer of the dentate gyrus in hippocampus (PubMed:9334400). Detected in neurons in the deep cerebellar nucleus (PubMed:23318870). Detected in globus pallidus and pars reticulata of the substantia nigra (PubMed:9334400). Detected in cerebellum (PubMed:23318870). Detected at axon terminal plexuses of cerebellar granule cells (PubMed:9334400). Detected in the juxtaparanodal region of nodes of Ranvier in cerebellum (at protein level) (PubMed:9334400). Detected in mesenteric arteries (PubMed:15618540). {ECO:0000269|PubMed:15618540, ECO:0000269|PubMed:21357749, ECO:0000269|PubMed:23318870, ECO:0000269|PubMed:9334400}.

Domain: In contrast to KCNAB1, the shorter N-terminal domain of KCNAB2 cannot mediate closure of delayed rectifier potassium channels by physically obstructing the pore. {ECO:0000250|UniProtKB:Q13303}.

Ptm: Phosphorylated by PRKCZ; may be regulated by incorporation in a complex composed of PRKCZ and SQSTM1. {ECO:0000269|PubMed:10477520}.

Similarity: Belongs to the shaker potassium channel beta subunit family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Rattus norvegicus (Rat).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus.



Function:		Cytoplasmic potassium channel subunit that modulates the characteristics of the channel-forming alpha-subunits (PubMed:9763623, PubMed:21357749). Contributes to the regulation of nerve signaling, and prevents neuronal hyperexcitability (By similarity). Promotes expression of the pore-forming alpha subunits at the cell membrane, and thereby increases channel activity (PubMed:10896669, PubMed:16770729, PubMed:18003609, PubMed:21357749). Promotes potassium channel closure via a mechanism that does not involve physical obstruction of the channel pore (PubMed:21357749). Modulates the functional properties of KCNA4 (PubMed:9763623). Modulates the functional properties of KCNA5 (By similarity). Enhances KCNB2 channel activity (By similarity). Binds NADPH and has NADPH-dependent aldoketoreductase activity (PubMed:18672894, PubMed:21209188). Has broad substrate specificity and can catalyze the reduction of methylglyoxal, 9,10-phenanthrenequinone, prostaglandin J2, 4-nitrobenzaldehyde, 4-nitroacetophenone and 4-oxo- trans-2-nonenal (in vitro) (PubMed:18672894). {ECO:0000250\|UniProtKB:P62482, ECO:0000269\|PubMed:10896669, ECO:0000269\|PubMed:18003609, ECO:0000269\|PubMed:18672894, ECO:0000269\|PubMed:21209188, ECO:0000269\|PubMed:21357749, ECO:0000269\|PubMed:9763623}.


Biophysicochemical properties:		pH dependence: Optimum pH is 7.2-7.4.;
Subunit:		Homotetramer (PubMed:10884227, PubMed:16002581, PubMed:18004376, PubMed:18806782, PubMed:20534430, PubMed:20360102, PubMed:23705070). Interaction with tetrameric potassium channel alpha subunits gives rise to a heterooctamer (PubMed:10884227, PubMed:16002581, PubMed:18004376, PubMed:18806782, PubMed:20534430, PubMed:20360102, PubMed:23705070). Identified in potassium channel complexes containing KCNA1, KCNA2, KCNA4, KCNA5, KCNA6, KCNAB1 and KCNAB2 (PubMed:9334400). Interacts with KCNA1 (PubMed:23318870, PubMed:10884227). Interacts with KCNA2 (PubMed:18003609, PubMed:21357749, PubMed:23318870, PubMed:16002581, PubMed:18004376, PubMed:18806782, PubMed:20534430, PubMed:20360102, PubMed:23705070). Interacts with KCNA4 and KCND3 (PubMed:12860406). Interacts with KCNA5 (By similarity). Interacts with KCNB2 (By similarity). Interacts (in unphosphorylated form) with MAPRE1 (PubMed:21357749). Forms a ternary complex with SQSTM1 and PRKCZ (PubMed:10477520). {ECO:0000250\|UniProtKB:P62482, ECO:0000269\|PubMed:10477520, ECO:0000269\|PubMed:10884227, ECO:0000269\|PubMed:12860406, ECO:0000269\|PubMed:16002581, ECO:0000269\|PubMed:18003609, ECO:0000269\|PubMed:18004376, ECO:0000269\|PubMed:18806782, ECO:0000269\|PubMed:20360102, ECO:0000269\|PubMed:20534430, ECO:0000269\|PubMed:21357749, ECO:0000269\|PubMed:23318870, ECO:0000269\|PubMed:23705070, ECO:0000269\|PubMed:9334400, ECO:0000269\|PubMed:9763623}.
Subcellular location:		Cytoplasm {ECO:0000269\|PubMed:21357749}. Membrane {ECO:0000269\|PubMed:21357749, ECO:0000269\|PubMed:9334400}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Cell membrane {ECO:0000269\|PubMed:23318870}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Cell projection, axon {ECO:0000269\|PubMed:21357749, ECO:0000269\|PubMed:9334400}. Cell junction, synapse, synaptosome {ECO:0000269\|PubMed:21357749}. Cytoplasm, cytoskeleton {ECO:0000269\|PubMed:21357749}. Note=Recruited to the cytoplasmic side of the cell membrane via its interaction with pore-forming potassium channel alpha subunits. Associates with microtubules when unphosphorylated (PubMed:21357749). {ECO:0000269\|PubMed:21357749, ECO:0000305}.
Tissue specificity:		Detected in brain (PubMed:9334400, PubMed:21357749). Detected in the middle third of the molecular layer of the dentate gyrus in hippocampus (PubMed:9334400). Detected in neurons in the deep cerebellar nucleus (PubMed:23318870). Detected in globus pallidus and pars reticulata of the substantia nigra (PubMed:9334400). Detected in cerebellum (PubMed:23318870). Detected at axon terminal plexuses of cerebellar granule cells (PubMed:9334400). Detected in the juxtaparanodal region of nodes of Ranvier in cerebellum (at protein level) (PubMed:9334400). Detected in mesenteric arteries (PubMed:15618540). {ECO:0000269\|PubMed:15618540, ECO:0000269\|PubMed:21357749, ECO:0000269\|PubMed:23318870, ECO:0000269\|PubMed:9334400}.
Domain:		In contrast to KCNAB1, the shorter N-terminal domain of KCNAB2 cannot mediate closure of delayed rectifier potassium channels by physically obstructing the pore. {ECO:0000250\|UniProtKB:Q13303}.
Ptm:		Phosphorylated by PRKCZ; may be regulated by incorporation in a complex composed of PRKCZ and SQSTM1. {ECO:0000269\|PubMed:10477520}.
Similarity:		Belongs to the shaker potassium channel beta subunit family. {ECO:0000305}.