 |
PDBsum entry 4jkw
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Immune system
|
PDB id
|
|
|
|
4jkw
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
PDB id:
|
|
|
|
| Name: |
|
Immune system
|
|
|
Title:
|
|
Structure of the extracellular domain of butyrophilin btn3a1 in complex with isopentenyl pyrophosphate (ipp)
|
|
Structure:
|
|
Butyrophilin subfamily 3 member a1. Chain: a. Fragment: unp residues 28-143. Engineered: yes. Mutation: yes
|
|
Source:
|
|
Homo sapiens. Human. Organism_taxid: 9606. Gene: bt3.2, btf3, btf4, btf5, btn3a1, btn3a2, butyrophilin subfamily 3 member a1. Expressed in: escherichia coli. Expression_system_taxid: 469008.
|
|
Resolution:
|
|
|
2.01Å
|
R-factor:
|
0.231
|
R-free:
|
0.268
|
|
|
Authors:
|
|
A.Kumar,L.Mori,G.De Libero
|
|
Key ref:
|
|
S.Vavassori
et al.
(2013).
Butyrophilin 3A1 binds phosphorylated antigens and stimulates human γδ T cells.
Nat Immunol,
14,
908-916.
PubMed id:
DOI:
|
|
|
Date:
|
|
|
12-Mar-13
|
Release date:
|
24-Jul-13
|
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
|
Headers
|
|
|
|
References
|
|
|
|
|
|
|
|
O00481
(BT3A1_HUMAN) -
Butyrophilin subfamily 3 member A1 from Homo sapiens
|
|
|
|
Seq:
Struc:
|
|
|
|
513 a.a.
119 a.a.*
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Key: |
 |
PfamA domain |
|
|
 |
Secondary structure |
|
 |
CATH domain |
|
|
*
PDB and UniProt seqs differ
at 5 residue positions (black
crosses)
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
| |
|
DOI no:
|
Nat Immunol
14:908-916
(2013)
|
|
PubMed id:
|
|
|
|
|
|
| |
|
Butyrophilin 3A1 binds phosphorylated antigens and stimulates human γδ T cells.
|
|
S.Vavassori,
A.Kumar,
G.S.Wan,
G.S.Ramanjaneyulu,
M.Cavallari,
S.El Daker,
T.Beddoe,
A.Theodossis,
N.K.Williams,
E.Gostick,
D.A.Price,
D.U.Soudamini,
K.K.Voon,
M.Olivo,
J.Rossjohn,
L.Mori,
G.De Libero.
|
|
|
|
|
| |
ABSTRACT
|
|
|
|
| |
|
|
Human T cells that express a T cell antigen receptor (TCR) containing γ-chain
variable region 9 and δ-chain variable region 2 (Vγ9Vδ2) recognize
phosphorylated prenyl metabolites as antigens in the presence of
antigen-presenting cells but independently of major histocompatibility complex
(MHC), the MHC class I-related molecule MR1 and antigen-presenting CD1
molecules. Here we used genetic approaches to identify the molecule that binds
and presents phosphorylated antigens. We found that the butyrophilin BTN3A1
bound phosphorylated antigens with low affinity, at a stoichiometry of 1:1, and
stimulated mouse T cells with transgenic expression of a human Vγ9Vδ2 TCR. The
structures of the BTN3A1 distal domain in complex with host- or microbe-derived
phosphorylated antigens had an immunoglobulin-like fold in which the antigens
bound in a shallow pocket. Soluble Vγ9Vδ2 TCR interacted specifically with
BTN3A1-antigen complexes. Accordingly, BTN3A1 represents an antigen-presenting
molecule required for the activation of Vγ9Vδ2 T cells.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
|
Links
