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PDBsum entry 4jj0
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Electron transport
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PDB id
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4jj0
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References listed in PDB file
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Key reference
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Title
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Structural insight into magnetochrome-Mediated magnetite biomineralization.
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Authors
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M.I.Siponen,
P.Legrand,
M.Widdrat,
S.R.Jones,
W.J.Zhang,
M.C.Chang,
D.Faivre,
P.Arnoux,
D.Pignol.
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Ref.
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Nature, 2013,
502,
681-684.
[DOI no: ]
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PubMed id
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Abstract
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Magnetotactic bacteria align along the Earth's magnetic field using an organelle
called the magnetosome, a biomineralized magnetite (Fe(II)Fe(III)2O4) or
greigite (Fe(II)Fe(III)2S4) crystal embedded in a lipid vesicle. Although the
need for both iron(II) and iron(III) is clear, little is known about the
biological mechanisms controlling their ratio. Here we present the structure of
the magnetosome-associated protein MamP and find that it is built on a unique
arrangement of a self-plugged PDZ domain fused to two magnetochrome domains,
defining a new class of c-type cytochrome exclusively found in magnetotactic
bacteria. Mutational analysis, enzyme kinetics, co-crystallization with iron(II)
and an in vitro MamP-assisted magnetite production assay establish MamP as an
iron oxidase that contributes to the formation of iron(III) ferrihydrite
eventually required for magnetite crystal growth in vivo. These results
demonstrate the molecular mechanisms of iron management taking place inside the
magnetosome and highlight the role of magnetochrome in iron biomineralization.
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