PDBsum entry 4je3

Cell cycle

PDB id: 4je3

Contents

Protein chains

244 a.a.

77 a.a.

Waters ×136

PDB id:

4je3

Name:

Cell cycle

Title:

An iml3-chl4 heterodimer links the core centromere to factors required for accurate chromosome segregation

Structure:

Central kinetochore subunit iml3. Chain: a. Synonym: increased minichromosome loss protein 3, minichromosome maintenance protein 19. Engineered: yes. Central kinetochore subunit chl4. Chain: b. Fragment: unp residues 361-458. Synonym: chromosome loss protein 4, chromosome transmission fidelity

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Gene: iml3, mcm19, ybr107c, ybr0836. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: chl4, ctf17, mcm17, ydr254w, yd9320a.04. Expression_system_taxid: 562

Resolution:

2.28Å R-factor: 0.186 R-free: 0.211

Authors:

S.M.Hinshaw,S.C.Harrison

Key ref:

S.M.Hinshaw and S.C.Harrison (2013). An Iml3-Chl4 heterodimer links the core centromere to factors required for accurate chromosome segregation. Cell Rep, 5, 29-36. PubMed id: 24075991 DOI: 10.1016/j.celrep.2013.08.036

Date:

26-Feb-13 Release date: 16-Oct-13

Protein chain

P38265  (CENPL_YEAST) -  Inner kinetochore subunit IML3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 245 a.a.

Struc: 244 a.a.

Protein chain

P38907  (CENPN_YEAST) -  Inner kinetochore subunit CHL4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 458 a.a.

Struc: 77 a.a.

Enzyme reactions

• Enzyme class: Chains A, B: E.C.?

DOI no: 10.1016/j.celrep.2013.08.036

Cell Rep 5:29-36 (2013)

PubMed id: 24075991

An Iml3-Chl4 heterodimer links the core centromere to factors required for accurate chromosome segregation.

S.M.Hinshaw, S.C.Harrison.

ABSTRACT

Accurate segregation of genetic material in eukaryotes relies on the kinetochore, a multiprotein complex that connects centromeric DNA with microtubules. In yeast and humans, two proteins-Mif2/CENP-C and Chl4/CNEP-N-interact with specialized centromeric nucleosomes and establish distinct but cross-connecting axes of chromatin-microtubule linkage. Proteins recruited by Chl4/CENP-N include a subset that regulates chromosome transmission fidelity. We show that Chl4 and a conserved member of this subset, Iml3, both from Saccharomyces cerevisiae, form a stable protein complex that interacts with Mif2 and Sgo1. We have determined the structures of an Iml3 homodimer and an Iml3-Chl4 heterodimer, which suggest a mechanism for regulating the assembly of this functional axis of the kinetochore. We propose that at the core centromere, the Chl4-Iml3 complex participates in recruiting factors, such as Sgo1, that influence sister chromatid cohesion and encourage sister kinetochore biorientation.