|
|
||
|
|
|
|
|
UniProt functional annotation for Q4D3W2 | ||
|
|
|
|
|
|
||
| UniProt code: Q4D3W2. |
|
||
| Organism: | |
Trypanosoma cruzi (strain CL Brener). |
| Taxonomy: | |
Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina; Trypanosomatida; Trypanosomatidae; Trypanosoma; Schizotrypanum. |
|
||
|
||
| Function: | |
Catalyzes the conversion of dihydroorotate to orotate with fumarate as the electron acceptor. Molecular oxygen can replace fumarate in vitro. |
|
||
| Catalytic activity: | |
Reaction=(S)-dihydroorotate + fumarate = orotate + succinate; Xref=Rhea:RHEA:30059, ChEBI:CHEBI:29806, ChEBI:CHEBI:30031, ChEBI:CHEBI:30839, ChEBI:CHEBI:30864; EC=1.3.98.1; |
| Cofactor: | |
Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000269|PubMed:18808149}; Note=Binds 1 FMN per subunit. {ECO:0000269|PubMed:18808149}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=57.3 uM for dihydroorotate {ECO:0000269|PubMed:16172019}; KM=62.2 uM for fumarate {ECO:0000269|PubMed:16172019}; pH dependence: Optimum pH is 7. {ECO:0000269|PubMed:16172019}; Temperature dependence: Optimum temperature is 37 degrees Celsius. {ECO:0000269|PubMed:16172019}; |
| Pathway: | |
Pyrimidine metabolism; UMP biosynthesis via de novo pathway. |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:18302934}. |
| Subcellular location: | |
Cytoplasm {ECO:0000250}. |
| Similarity: | |
Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.