PDBsum entry 4jbw

Transport protein

PDB id: 4jbw

Contents

Protein chains

492 a.a.

286 a.a.

370 a.a.

150 a.a.

Ligands

PGV ×2

PDB id:

4jbw

Name:

Transport protein

Title:

Crystal structure of e. Coli maltose transporter malfgk2 in complex with its regulatory protein eiiaglc

Structure:

Maltose transport system permease protein malf. Chain: f, h. Engineered: yes. Maltose transport system permease protein malg. Chain: g, i. Engineered: yes. Maltose/maltodextrin import atp-binding protein malk. Chain: a, b, c, d. Engineered: yes.

Source:

Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: malf, b4033, jw3993. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: malg, b4032, jw3992. Gene: malk, b4035, jw3995. Gene: crr, gsr, iex, tgs, tred, b2417, jw2410.

Resolution:

3.91Å R-factor: 0.229 R-free: 0.281

Authors:

S.Chen,M.L.Oldham,A.L.Davidson,J.Chen

Key ref:

S.Chen et al. (2013). Carbon catabolite repression of the maltose transporter revealed by X-ray crystallography. Nature, 499, 364-368. PubMed id: 23770568 DOI: 10.1038/nature12232

Date:

20-Feb-13 Release date: 12-Jun-13

Protein chains

P02916  (MALF_ECOLI) -  Maltose/maltodextrin transport system permease protein MalF from Escherichia coli (strain K12)

Seq: 514 a.a.

Struc: 492 a.a.

Protein chains

P68183  (MALG_ECOLI) -  Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12)

Seq: 296 a.a.

Struc: 286 a.a.

Protein chains

P68187  (MALK_ECOLI) -  Maltose/maltodextrin import ATP-binding protein MalK from Escherichia coli (strain K12)

Seq: 371 a.a.

Struc: 370 a.a.

Protein chains

P69783  (PTGA_ECOLI) -  PTS system glucose-specific EIIA component from Escherichia coli (strain K12)

Seq: 169 a.a.

Struc: 150 a.a.

Enzyme reactions

• Enzyme class 2: Chains A, B, C, D: E.C.7.5.2.1 - ABC-type maltose transporter.
• Reaction: D-maltose(out) + ATP + H2O = D-maltose(in) + ADP + phosphate + H⁺
• Enzyme class 3: Chains M, N, O, P: E.C.2.7.1.- - ?????

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1038/nature12232

Nature 499:364-368 (2013)

PubMed id: 23770568

Carbon catabolite repression of the maltose transporter revealed by X-ray crystallography.

S.Chen, M.L.Oldham, A.L.Davidson, J.Chen.

ABSTRACT

No abstract given.