PDBsum entry 4j8v

Structural protein/DNA

PDB id: 4j8v

Contents

Protein chains

97 a.a.

82 a.a.

106 a.a.

95 a.a.

87 a.a.

DNA/RNA

Ligands

SO4 ×3

RU7 ×2

Metals

_MG

PDB id:

4j8v

Name:

Structural protein/DNA

Title:

X-ray structure of ncp145 with bound chlorido(eta-6-p-cymene)(n- phenyl-2-pyridinecarbothioamide)ruthenium(ii)

Structure:

Histone h3.2. Chain: a, e. Engineered: yes. Histone h4. Chain: b, f. Engineered: yes. Histone h2a. Chain: c, g. Engineered: yes.

Source:

Xenopus laevis. Clawed frog,common platanna,platanna. Organism_taxid: 8355. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: hist1h2aj, loc494591. Synthetic: yes. Synthetic construct. Organism_taxid: 32630.

Resolution:

2.58Å R-factor: 0.253 R-free: 0.273

Authors:

Z.Adhireksan,C.A.Davey

Key ref:

S.M.Meier et al. (2013). Novel metal(ii) arene 2-Pyridinecarbothioamides: a ra to orally active organometallic anticancer agents. Chem sci, 4, 1837.

Date:

15-Feb-13 Release date: 08-May-13

Protein chains

P84233  (H32_XENLA) -  Histone H3.2 from Xenopus laevis

Seq: 136 a.a.

Struc: 97 a.a.*

Protein chain

P62799  (H4_XENLA) -  Histone H4 from Xenopus laevis

Seq: 103 a.a.

Struc: 82 a.a.

Protein chains

P06897  (H2A1_XENLA) -  Histone H2A type 1 from Xenopus laevis

Seq: 130 a.a.

Struc: 106 a.a.*

Protein chains

P02281  (H2B11_XENLA) -  Histone H2B 1.1 from Xenopus laevis

Seq: 126 a.a.

Struc: 95 a.a.*

Protein chain

P62799  (H4_XENLA) -  Histone H4 from Xenopus laevis

Seq: 103 a.a.

Struc: 87 a.a.

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

DNA/RNA chains

A-T-C-A-A-T-A-T-C-C-A-C-C-T-G-C-A-G-A-T-A-C-T-A-C-C-A-A-A-A-G-T-G-T-A-T-T-T-G- 145 bases

A-T-C-A-A-T-A-T-C-C-A-C-C-T-G-C-A-G-A-T-A-C-T-A-C-C-A-A-A-A-G-T-G-T-A-T-T-T-G- 145 bases

Enzyme reactions

• Enzyme class: Chains A, B, C, D, E, F, G, H: E.C.?