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PDBsum entry 4ijd
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PDB id:
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Transferase
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Title:
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Crystal structure of methyltransferase domain of human pr domain- containing protein 9
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Structure:
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Histone-lysine n-methyltransferase prdm9. Chain: a, b. Fragment: unp residues 195-415. Synonym: pr domain zinc finger protein 9, pr domain-containing protein 9. Engineered: yes
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: prdm9, pfm6. Expressed in: escherichia coli. Expression_system_taxid: 511693.
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Resolution:
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2.15Å
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R-factor:
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0.199
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R-free:
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0.264
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Authors:
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A.Dong,L.Dombrovski,Y.Li,W.Tempel,C.Bountra,C.H.Arrowsmith, A.M.Edwards,P.J.Brown,H.Wu,Structural Genomics Consortium (Sgc)
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Key ref:
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L.Dombrovski
et al.
Crystal structure of methyltransferase domain of huma domain-Containing protein 9.
To be published,
.
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Date:
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21-Dec-12
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Release date:
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13-Feb-13
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PROCHECK
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Headers
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References
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Q9NQV7
(PRDM9_HUMAN) -
Histone-lysine N-methyltransferase PRDM9 from Homo sapiens
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Seq:
Struc:
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894 a.a.
215 a.a.
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Key: |
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Secondary structure |
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CATH domain |
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Enzyme class 1:
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E.C.2.1.1.-
- ?????
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Enzyme class 2:
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E.C.2.1.1.354
- [histone H3]-lysine(4) N-trimethyltransferase.
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Reaction:
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L-lysyl4-[histone H3] + 3 S-adenosyl-L-methionine = N6,N6,N6- trimethyl-L-lysyl4-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H+
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L-lysyl(4)-[histone H3]
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+
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3
×
S-adenosyl-L-methionine
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=
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N(6),N(6),N(6)- trimethyl-L-lysyl(4)-[histone H3]
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+
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3
×
S-adenosyl-L-homocysteine
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+
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3
×
H(+)
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Enzyme class 3:
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E.C.2.1.1.355
- [histone H3]-lysine(9) N-trimethyltransferase.
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Reaction:
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L-lysyl9-[histone H3] + 3 S-adenosyl-L-methionine = N6,N6,N6- trimethyl-L-lysyl9-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H+
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L-lysyl(9)-[histone H3]
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+
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3
×
S-adenosyl-L-methionine
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=
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N(6),N(6),N(6)- trimethyl-L-lysyl(9)-[histone H3]
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+
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3
×
S-adenosyl-L-homocysteine
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+
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3
×
H(+)
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Enzyme class 4:
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E.C.2.1.1.359
- [histone H3]-lysine(36) N-trimethyltransferase.
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Reaction:
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L-lysyl36-[histone H3] + 3 S-adenosyl-L-methionine = N6,N6,N6- trimethyl-L-lysyl36-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H+
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L-lysyl(36)-[histone H3]
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+
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3
×
S-adenosyl-L-methionine
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=
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N(6),N(6),N(6)- trimethyl-L-lysyl(36)-[histone H3]
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+
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3
×
S-adenosyl-L-homocysteine
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+
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3
×
H(+)
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Enzyme class 5:
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E.C.2.1.1.361
- [histone H4]-lysine(20) N-methyltransferase.
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Reaction:
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L-lysyl20-[histone H4] + S-adenosyl-L-methionine = N6-methyl-L- lysyl20-[histone H4] + S-adenosyl-L-homocysteine + H+
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L-lysyl(20)-[histone H4]
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+
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3
×
S-adenosyl-L-methionine
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=
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N(6)-methyl-L- lysyl(20)-[histone H4]
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+
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3
×
S-adenosyl-L-homocysteine
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+
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3
×
H(+)
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Enzyme class 6:
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E.C.2.1.1.362
- [histone H4]-N-methyl-L-lysine(20) N-methyltransferase.
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Reaction:
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N6-methyl-L-lysyl20-[histone H4] + S-adenosyl-L-methionine = N6,N6-dimethyl-L-lysyl20-[histone H4] + S-adenosyl-L-homocysteine + H+
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N(6)-methyl-L-lysyl(20)-[histone H4]
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+
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3
×
S-adenosyl-L-methionine
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=
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N(6),N(6)-dimethyl-L-lysyl(20)-[histone H4]
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+
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3
×
S-adenosyl-L-homocysteine
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+
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3
×
H(+)
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Links
