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UniProt functional annotation for O94609 | ||
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| UniProt code: O94609. |
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| Organism: | |
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. |
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| Function: | |
Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. {ECO:0000269|PubMed:23416107}. |
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| Catalytic activity: | |
Reaction=ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]- L-cysteine.; EC=6.2.1.45; Evidence={ECO:0000269|PubMed:23416107}; |
| Pathway: | |
Protein modification; protein ubiquitination. {ECO:0000269|PubMed:23416107}. |
| Subunit: | |
Monomer. Interacts with the E2 ubiquitin-conjugating enzyme ubc4. {ECO:0000269|PubMed:23416107}. |
| Subcellular location: | |
Cytoplasm. Nucleus. |
| Miscellaneous: | |
There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. {ECO:0000250|UniProtKB:P22515}. |
| Similarity: | |
Belongs to the ubiquitin-activating E1 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.