PDBsum entry 4hxd

Hydrolase/viral protein

PDB id: 4hxd

Contents

Protein chains

75 a.a.

157 a.a.

Ligands

4LJ ×2

SO4 ×3

Waters ×46

PDB id:

4hxd

Name:

Hydrolase/viral protein

Title:

Diversity of ubiquitin and isg15 specificity amongst nairoviruses viral ovarian tumor domain proteases

Structure:

Polyubiquitin-c. Chain: a, c. Synonym: ubiquitin. Engineered: yes. RNA-directed RNA polymerase l. Chain: b, d. Synonym: protein l, large structural protein, replicase, transcriptase, ubiquitin thioesterase, RNA-directed RNA polymerase. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ubc. Expressed in: escherichia coli. Expression_system_taxid: 469008. Dugbe virus (isolate ard44313). Dugv. Organism_taxid: 766194.

Resolution:

2.85Å R-factor: 0.214 R-free: 0.278

Authors:

G.C.Capodagli,S.D.Pegan

Key ref:

G.C.Capodagli et al. (2013). Diversity of ubiquitin and ISG15 specificity among nairoviruses' viral ovarian tumor domain proteases. J Virol, 87, 3815-3827. PubMed id: 23345508 DOI: 10.1128/JVI.03252-12

Date:

09-Nov-12 Release date: 13-Feb-13

Protein chains

P0CG48  (UBC_HUMAN) -  Polyubiquitin-C from Homo sapiens

Seq: 685 a.a.

Struc: 75 a.a.

Protein chains

Q66431  (L_DUGBA) -  RNA-directed RNA polymerase L from Dugbe virus (isolate ArD44313)

Seq: 4036 a.a.

Struc: 157 a.a.

Enzyme reactions

• Enzyme class 2: Chains A, B, C, D: E.C.3.4.19.12 - ubiquitinyl hydrolase 1.
• Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
• Enzyme class 3: Chains B, D: E.C.2.7.7.48 - RNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
• Enzyme class 4: Chains B, D: E.C.3.1.-.-
• Enzyme class 5: Chains B, D: E.C.3.4.22.- - ?????

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1128/JVI.03252-12

J Virol 87:3815-3827 (2013)

PubMed id: 23345508

Diversity of ubiquitin and ISG15 specificity among nairoviruses' viral ovarian tumor domain proteases.

G.C.Capodagli, M.K.Deaton, E.A.Baker, R.J.Lumpkin, S.D.Pegan.

ABSTRACT

No abstract given.