UniProt functional annotation for Q9RY80



	UniProt functional annotation for Q9RY80

UniProt code: Q9RY80.

Organism: Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422).

Taxonomy: Bacteria; Deinococcus-Thermus; Deinococci; Deinococcales; Deinococcaceae; Deinococcus.

Function: ssDNA-binding protein that contributes to the ionizing radiation resistance of D.radiodurans. Plays a role in DNA repair and genome reconstitution in a RecA-independent process. Required for recovery from severe genomic fragmentation as a result of exposure to severe levels of ionizing radiation. Binds ssDNA but not dsDNA. Stimulates annealing of complementary ssDNA. Does not complement an ssb disruption. {ECO:0000269|PubMed:15454524, ECO:0000269|PubMed:19515845, ECO:0000269|PubMed:20451472, ECO:0000269|PubMed:23951213}.

Subunit: Homopentamer arranged in a ring-structure; DNA binds between subunits and along the top of the ring. The pentamers self-associate to coat ssDNA in higher-ordered structures; oligomerization facilitates the assembly of extended nucleoprotein complexes. Self-assembly does not however require ssDNA-binding. Interacts with SSB. {ECO:0000269|PubMed:19515845, ECO:0000269|PubMed:20451472, ECO:0000269|PubMed:23975200}.

Induction: Induced to high levels following extreme ionizing radiation exposure. Also highly induced in response to desiccation stress. {ECO:0000269|PubMed:15454524}.

Domain: Contains a novel ssDNA-binding fold, which is structurally and topologically distinct from the OB-fold universally found in standard SSB proteins. The disordered C-terminus of DdrB may mediate interactions with other proteins important for DNA damage recovery (By similarity). {ECO:0000250}.

Mass spectrometry: Mass=25236.7; Method=SELDI; Note=Tagged N-terminally with 6 His residues.; Evidence={ECO:0000269|PubMed:20451472};

Disruption phenotype: Cells lacking this gene show a normal growth rate, do not exhibit a decrease in the efficiency of natural transformation, but display a reduced capacity to survive ionizing radiation when exposed at doses superior to 2.5 kGy. DNA repair following irradiations is slower. Cannot be complemented by ssb. A double recA-ddrB disruption shows no signs of DNA repair 24 hours after irradiation. {ECO:0000269|PubMed:15454524, ECO:0000269|PubMed:20451472, ECO:0000269|PubMed:23951213}.

Sequence caution: Sequence=AAF09667.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422).
Taxonomy:		Bacteria; Deinococcus-Thermus; Deinococci; Deinococcales; Deinococcaceae; Deinococcus.



Function:		ssDNA-binding protein that contributes to the ionizing radiation resistance of D.radiodurans. Plays a role in DNA repair and genome reconstitution in a RecA-independent process. Required for recovery from severe genomic fragmentation as a result of exposure to severe levels of ionizing radiation. Binds ssDNA but not dsDNA. Stimulates annealing of complementary ssDNA. Does not complement an ssb disruption. {ECO:0000269\|PubMed:15454524, ECO:0000269\|PubMed:19515845, ECO:0000269\|PubMed:20451472, ECO:0000269\|PubMed:23951213}.


Subunit:		Homopentamer arranged in a ring-structure; DNA binds between subunits and along the top of the ring. The pentamers self-associate to coat ssDNA in higher-ordered structures; oligomerization facilitates the assembly of extended nucleoprotein complexes. Self-assembly does not however require ssDNA-binding. Interacts with SSB. {ECO:0000269\|PubMed:19515845, ECO:0000269\|PubMed:20451472, ECO:0000269\|PubMed:23975200}.
Induction:		Induced to high levels following extreme ionizing radiation exposure. Also highly induced in response to desiccation stress. {ECO:0000269\|PubMed:15454524}.
Domain:		Contains a novel ssDNA-binding fold, which is structurally and topologically distinct from the OB-fold universally found in standard SSB proteins. The disordered C-terminus of DdrB may mediate interactions with other proteins important for DNA damage recovery (By similarity). {ECO:0000250}.
Mass spectrometry:		Mass=25236.7; Method=SELDI; Note=Tagged N-terminally with 6 His residues.; Evidence={ECO:0000269\|PubMed:20451472};
Disruption phenotype:		Cells lacking this gene show a normal growth rate, do not exhibit a decrease in the efficiency of natural transformation, but display a reduced capacity to survive ionizing radiation when exposed at doses superior to 2.5 kGy. DNA repair following irradiations is slower. Cannot be complemented by ssb. A double recA-ddrB disruption shows no signs of DNA repair 24 hours after irradiation. {ECO:0000269\|PubMed:15454524, ECO:0000269\|PubMed:20451472, ECO:0000269\|PubMed:23951213}.
Sequence caution:		Sequence=AAF09667.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};