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UniProt functional annotation for A9CEQ8 | ||
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| UniProt code: A9CEQ8. |
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| Organism: | |
Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)). |
| Taxonomy: | |
Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium; Agrobacterium tumefaciens complex. |
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| Function: | |
Catalyzes the ring opening of D-galactaro-1,4-lactone to yield 5-keto-4-deoxy-D-glucarate (KDG) via a beta-elimination reaction. This is a step in the oxidative degradation pathway of D-galacturonate, which allows A.tumefaciens to utilize D-galacturonate as a sole carbon source. To a lesser extent, can also use D-glucaro-1,4-lactone as substrate to produce KDG, but cannot use D-galactaro-1,5-lactone, D- glucaro-6,3-lactone and linear D-glucarate. {ECO:0000269|PubMed:22493433, ECO:0000269|PubMed:24450804}. |
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| Catalytic activity: | |
Reaction=D-glucaro-1,4-lactone = 5-dehydro-4-deoxy-D-glucarate + H(+); Xref=Rhea:RHEA:45604, ChEBI:CHEBI:15378, ChEBI:CHEBI:42819, ChEBI:CHEBI:85336; EC=5.5.1.27; Evidence={ECO:0000269|PubMed:22493433, ECO:0000269|PubMed:24450804}; |
| Catalytic activity: | |
Reaction=D-galactaro-1,4-lactone = 5-dehydro-4-deoxy-D-glucarate + H(+); Xref=Rhea:RHEA:45600, ChEBI:CHEBI:15378, ChEBI:CHEBI:42819, ChEBI:CHEBI:85317; EC=5.5.1.27; Evidence={ECO:0000269|PubMed:22493433, ECO:0000269|PubMed:24450804}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:22493433}; |
| Biophysicochemical properties: | |
pH dependence: Optimum pH is 8.5. {ECO:0000269|PubMed:22493433}; |
| Pathway: | |
Carbohydrate acid metabolism; D-galacturonate degradation via prokaryotic oxidative pathway. {ECO:0000269|PubMed:24450804}. |
| Subunit: | |
Homooctamer. {ECO:0000269|PubMed:22493433, ECO:0000269|Ref.5}. |
| Similarity: | |
Belongs to the mandelate racemase/muconate lactonizing enzyme family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.