 |
PDBsum entry 4hk1
|
|
|
|
References listed in PDB file
|
 |
|
Key reference
|
|
|
Title
|
|
Structure of pcna from drosophila melanogaster.
|
|
|
Authors
|
|
K.Wang,
Z.Shi,
M.Zhang,
D.Cheng.
|
|
|
Ref.
|
|
Acta Crystallogr Sect F Struct Biol Cryst Commun, 2013,
69,
387-392.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
Proliferating cell nuclear antigen (PCNA) plays essential roles in DNA
replication, DNA repair, cell-cycle regulation and chromatin metabolism. The
PCNA from Drosophila melanogaster (DmPCNA) was purified and crystallized. The
crystal of DmPCNA diffracted to 2.0 Å resolution and belonged to space group
H3, with unit-cell parameters a = b = 151.16, c = 38.28 Å. The structure of
DmPCNA was determined by molecular replacement. DmPCNA forms a symmetric
homotrimer in a head-to-tail manner. An interdomain connector loop (IDCL) links
the N- and C-terminal domains. Additionally, the N-terminal and C-terminal
domains contact each other through hydrophobic associations. Compared with human
PCNA, the IDCL of DmPCNA has conformational changes, which may explain their
difference in function. This work provides a structural basis for further
functional and evolutionary studies of PCNA.
|
|
|
|
|
|
|
