PDBsum entry 4hk1

Cell cycle

PDB id: 4hk1

Contents

Protein chain

243 a.a.

Waters ×115

PDB id:

4hk1

Name:

Cell cycle

Title:

Crystal structure of pcna from drosophila melanogaster

Structure:

Proliferating cell nuclear antigen. Chain: a. Synonym: pcna, cyclin, mutagen-sensitive 209 protein. Engineered: yes

Source:

Drosophila melanogaster. Fruit fly. Organism_taxid: 7227. Gene: mus209, pcna, cg9193. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.00Å R-factor: 0.231 R-free: 0.278

Authors:

K.Wang,Z.B.Shi,Z.C.Zhou

Key ref:

K.Wang et al. (2013). Structure of PCNA from Drosophila melanogaster. Acta Crystallogr Sect F Struct Biol Cryst Commun, 69, 387-392. PubMed id: 23545643 DOI: 10.1107/S1744309113004971

Date:

14-Oct-12 Release date: 10-Apr-13

Protein chain

P17917  (PCNA_DROME) -  Proliferating cell nuclear antigen from Drosophila melanogaster

Seq: 260 a.a.

Struc: 243 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.?

DOI no: 10.1107/S1744309113004971

Acta Crystallogr Sect F Struct Biol Cryst Commun 69:387-392 (2013)

PubMed id: 23545643

Structure of PCNA from Drosophila melanogaster.

K.Wang, Z.Shi, M.Zhang, D.Cheng.

ABSTRACT

Proliferating cell nuclear antigen (PCNA) plays essential roles in DNA replication, DNA repair, cell-cycle regulation and chromatin metabolism. The PCNA from Drosophila melanogaster (DmPCNA) was purified and crystallized. The crystal of DmPCNA diffracted to 2.0 Å resolution and belonged to space group H3, with unit-cell parameters a = b = 151.16, c = 38.28 Å. The structure of DmPCNA was determined by molecular replacement. DmPCNA forms a symmetric homotrimer in a head-to-tail manner. An interdomain connector loop (IDCL) links the N- and C-terminal domains. Additionally, the N-terminal and C-terminal domains contact each other through hydrophobic associations. Compared with human PCNA, the IDCL of DmPCNA has conformational changes, which may explain their difference in function. This work provides a structural basis for further functional and evolutionary studies of PCNA.