PDBsum entry 4hi8

Signaling protein

PDB id: 4hi8

Contents

Protein chains

170 a.a.

72 a.a.

Ligands

PO4

Metals

_ZN ×2

Waters ×467

PDB id:

4hi8

Name:

Signaling protein

Title:

Structure of integrin-linked kinase ankyrin repeat domain in complex with pinch1 lim1 domain collected at high energy, wavelength 0.32800

Structure:

Integrin-linked protein kinase. Chain: a. Fragment: ankyrin repeat domain. Synonym: 59 kda serine/threonine-protein kinase, ilk-1, ilk-2, p59ilk. Engineered: yes. Lim and senescent cell antigen-like-containing domain protein 1. Chain: b.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ilk, ilk1, ilk2. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: lims1, pinch, pinch1.

Resolution:

1.20Å R-factor: 0.145 R-free: 0.168

Authors:

A.L.Stiegler,J.Jakoncic,V.Stojanoff,B.P.Chiswell,D.A.Calderwood, T.J.Boggon

Key ref:

A.L.Stiegler et al. Structure of integrin-Linked kinase ankyrin repeat do complex with pinch1 lim1 domain collected at high ene wavelength 0.32800. To be published, .

Date:

11-Oct-12 Release date: 11-Dec-13

Protein chain

Q13418  (ILK_HUMAN) -  Scaffold protein ILK from Homo sapiens

Seq: 452 a.a.

Struc: 170 a.a.

Protein chain

P48059  (LIMS1_HUMAN) -  LIM and senescent cell antigen-like-containing domain protein 1 from Homo sapiens

Seq: 325 a.a.

Struc: 72 a.a.*

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site