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PDBsum entry 4ha9
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Oxidoreductase
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PDB id
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4ha9
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References listed in PDB file
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Key reference
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Title
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Structural and functional insights into saccharomyces cerevisiae riboflavin biosynthesis reductase rib7.
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Authors
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Z.Lv,
J.Sun,
Y.Liu.
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Ref.
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Plos One, 2013,
8,
e61249.
[DOI no: ]
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PubMed id
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Abstract
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Saccharomyces cerevisiae RIB7 (ScRIB7) is a potent target for anti-fungal agents
because of its involvement in the riboflavin biosynthesis pathway as a
NADPH-dependent reductase. However, the catalytic mechanism of riboflavin
biosynthesis reductase (RBSRs) is controversial, and enzyme structure
information is still lacking in eukaryotes. Here we report the crystal structure
of Saccharomyces cerevisiae RIB7 at 2.10 Å resolution and its complex with
NADPH at 2.35 Å resolution. ScRIB7 exists as a stable homodimer, and each
subunit consists of nine central β-sheets flanked by five helices, resembling
the structure of RIB7 homologues. A conserved G(76)-X-G(78)-Xn-G(181)-G(182)
motif is present at the NADPH pyrophosphate group binding site. Activity assays
confirmed the necessity of Thr79, Asp83, Glu180 and Gly182 for the activity of
ScRIB7. Substrate preference of ScRIB7 was altered by mutating one residue
(Thr35) to a Lysine, implying that ScRIB7 Thr35 and its corresponding residue, a
lysine in bacteria, are important in substrate-specific recognition.
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