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PDBsum entry 4h61
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Transcription
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PDB id
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4h61
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PDB id:
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| Name: |
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Transcription
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Title:
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Structure of the schizosaccharomyces pombe mediator subunit med6
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Structure:
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Mediator of RNA polymerase ii transcription subunit 6. Chain: a, b. Fragment: unp reisdues 10-180. Synonym: mediator complex subunit 6, RNA polymerase ii mediator complex protein pmc5. Engineered: yes
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Source:
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Schizosaccharomyces pombe. Fission yeast. Organism_taxid: 284812. Strain: 972h. Gene: med6, pmc5, spac1002.15c. Expressed in: escherichia coli. Expression_system_taxid: 469008.
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Resolution:
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2.70Å
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R-factor:
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0.183
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R-free:
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0.217
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Authors:
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L.Lariviere,C.Plaschka,M.Seizl,L.Wenzeck,F.Kurth,P.Cramer
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Key ref:
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L.Larivière
et al.
(2012).
Structure of the Mediator head module.
Nature,
492,
448-451.
PubMed id:
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Date:
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19-Sep-12
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Release date:
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31-Oct-12
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PROCHECK
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Headers
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References
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Q9US45
(MED6_SCHPO) -
Mediator of RNA polymerase II transcription subunit 6 from Schizosaccharomyces pombe (strain 972 / ATCC 24843)
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Seq:
Struc:
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216 a.a.
137 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 2 residue positions (black
crosses)
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Nature
492:448-451
(2012)
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PubMed id:
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Structure of the Mediator head module.
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L.Larivière,
C.Plaschka,
M.Seizl,
L.Wenzeck,
F.Kurth,
P.Cramer.
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ABSTRACT
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Gene transcription by RNA polymerase (Pol) II requires the coactivator complex
Mediator. Mediator connects transcriptional regulators and Pol II, and is linked
to human disease. Mediator from the yeast Saccharomyces cerevisiae has a
molecular mass of 1.4 megadaltons and comprises 25 subunits that form the
head, middle, tail and kinase modules. The head module constitutes one-half of
the essential Mediator core, and comprises the conserved subunits Med6, Med8,
Med11, Med17, Med18, Med20 and Med22. Recent X-ray analysis of the S. cerevisiae
head module at 4.3 Å resolution led to a partial architectural model with
three submodules called neck, fixed jaw and moveable jaw. Here we determine de
novo the crystal structure of the head module from the fission yeast
Schizosaccharomyces pombe at 3.4 Å resolution. Structure solution was enabled
by new structures of Med6 and the fixed jaw, and previous structures of the
moveable jaw and part of the neck, and required deletion of Med20. The S. pombe
head module resembles the head of a crocodile with eight distinct elements, of
which at least four are mobile. The fixed jaw comprises tooth and nose domains,
whereas the neck submodule contains a helical spine and one limb, with shoulder,
arm and finger elements. The arm and the essential shoulder contact other parts
of Mediator. The jaws and a central joint are implicated in interactions with
Pol II and its carboxy-terminal domain, and the joint is required for
transcription in vitro. The S. pombe head module structure leads to a revised
model of the S. cerevisiae module, reveals a high conservation and flexibility,
explains known mutations, and provides the basis for unravelling a central
mechanism of gene regulation.
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Links
