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PDBsum entry 4gz5
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PDB id:
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Transferase
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Title:
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Crystal structure of human o-glcnac transferase with udp-glcnac
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Structure:
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Udp-n-acetylglucosamine--peptide n- acetylglucosaminyltransferase 110 kda subunit. Chain: a, b, c, d. Synonym: o-glcnac transferase subunit p110, o-linked n- acetylglucosamine transferase 110 kda subunit, ogt. Engineered: yes
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: ogt. Expressed in: escherichia coli. Expression_system_taxid: 562
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Resolution:
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3.08Å
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R-factor:
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0.201
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R-free:
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0.229
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Authors:
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M.B.Lazarus,J.Jiang,T.M.Gloster,W.F.Zandberg,D.J.Vocadlo,S.Walker
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Key ref:
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M.B.Lazarus
et al.
(2012).
Structural snapshots of the reaction coordinate for O-GlcNAc transferase.
Nat Chem Biol,
8,
966-968.
PubMed id:
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Date:
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06-Sep-12
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Release date:
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31-Oct-12
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PROCHECK
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Headers
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References
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O15294
(OGT1_HUMAN) -
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit from Homo sapiens
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Seq:
Struc:
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1046 a.a.
700 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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Enzyme class:
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E.C.2.4.1.255
- protein O-GlcNAc transferase.
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Reaction:
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1.
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L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl- beta-D-glucosaminyl)-L-seryl-[protein] + UDP + H+
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2.
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L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O- (N-acetyl-beta-D-glucosaminyl)-L-threonyl-[protein] + UDP + H+
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L-seryl-[protein]
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+
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UDP-N-acetyl-alpha-D-glucosamine
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=
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3-O-(N-acetyl- beta-D-glucosaminyl)-L-seryl-[protein]
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+
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UDP
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+
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H(+)
Bound ligand (Het Group name = )
matches with 64.10% similarity
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L-threonyl-[protein]
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+
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UDP-N-acetyl-alpha-D-glucosamine
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=
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3-O- (N-acetyl-beta-D-glucosaminyl)-L-threonyl-[protein]
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+
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UDP
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+
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H(+)
Bound ligand (Het Group name = )
matches with 64.10% similarity
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Nat Chem Biol
8:966-968
(2012)
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PubMed id:
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Structural snapshots of the reaction coordinate for O-GlcNAc transferase.
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M.B.Lazarus,
J.Jiang,
T.M.Gloster,
W.F.Zandberg,
G.E.Whitworth,
D.J.Vocadlo,
S.Walker.
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ABSTRACT
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