PDBsum entry 4gz3

Transferase/peptide

PDB id

4gz3

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Contents

			Protein chains

					695 a.a.


					14 a.a.

			Ligands

					UDP ×2


					SO4 ×3


					0YT ×2

			Waters ×836

PDB id:

4gz3

Links

Name:

Transferase/peptide

Title:

Crystal structure of human o-glcnac transferase with udp and a thioglycopeptide

Structure:

Udp-n-acetylglucosamine--peptide n- acetylglucosaminyltransferase 110 kda subunit. Chain: a, c. Synonym: o-glcnac transferase subunit p110, o-linked n- acetylglucosamine transferase 110 kda subunit, ogt. Engineered: yes. Casein kinase ii subunit alpha. Chain: b, d. Synonym: ck ii alpha.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ogt. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: synthetic peptide

Resolution:

1.90Å

R-factor:

0.227

R-free:

0.250

Authors:

M.B.Lazarus,J.Jiang,T.M.Gloster,W.F.Zandberg,D.J.Vocadlo,S.Walker

Key ref:

M.B.Lazarus et al. (2012). Structural snapshots of the reaction coordinate for O-GlcNAc transferase. Nat Chem Biol, 8, 966-968. PubMed id: 23103939

Date:

05-Sep-12

Release date:

31-Oct-12

PROCHECK

	Headers

	References

Protein chains

O15294 (OGT1_HUMAN) - UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit from Homo sapiens

Seq: Struc:

Seq: Struc:

Seq: Struc:					1046 a.a. 695 a.a.

Protein chains

P68400 (CSK21_HUMAN) - Casein kinase II subunit alpha from Homo sapiens

Seq: Struc:					391 a.a. 14 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)



		Enzyme reactions

Enzyme class 1:

Chains A, C: E.C.2.4.1.255 - protein O-GlcNAc transferase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

1.	L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl- beta-D-glucosaminyl)-L-seryl-[protein] + UDP + H⁺
2.	L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O- (N-acetyl-beta-D-glucosaminyl)-L-threonyl-[protein] + UDP + H⁺

L-seryl-[protein]	+	UDP-N-acetyl-alpha-D-glucosamine	=	3-O-(N-acetyl- beta-D-glucosaminyl)-L-seryl-[protein]	+	UDP	+	H(+) Bound ligand (Het Group name = UDP) corresponds exactly

L-threonyl-[protein]	+	UDP-N-acetyl-alpha-D-glucosamine	=	3-O- (N-acetyl-beta-D-glucosaminyl)-L-threonyl-[protein]	+	UDP	+	H(+) Bound ligand (Het Group name = UDP) corresponds exactly

Enzyme class 2:

Chains B, D: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

1.	L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
2.	L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

L-seryl-[protein]	+	ATP	=	O-phospho-L-seryl-[protein] Bound ligand (Het Group name = UDP) matches with 79.31% similarity	+	ADP	+	H(+)

L-threonyl-[protein]	+	ATP	=	O-phospho-L-threonyl-[protein] Bound ligand (Het Group name = UDP) matches with 79.31% similarity	+	ADP	+	H(+)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

	Nat Chem Biol 8:966-968 (2012)
PubMed id: 23103939

Structural snapshots of the reaction coordinate for O-GlcNAc transferase.
M.B.Lazarus, J.Jiang, T.M.Gloster, W.F.Zandberg, G.E.Whitworth, D.J.Vocadlo, S.Walker.

ABSTRACT

No abstract given.