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UniProt functional annotation for Q8NB78 | ||
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| UniProt code: Q8NB78. |
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| Organism: | |
Homo sapiens (Human). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. |
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| Function: | |
Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4. {ECO:0000269|PubMed:23260659, ECO:0000269|PubMed:23357850}. |
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| Catalytic activity: | |
Reaction=2 A + 2 H2O + N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] = 2 AH2 + 2 formaldehyde + L-lysyl(4)-[histone H3]; Xref=Rhea:RHEA:60244, Rhea:RHEA-COMP:15540, Rhea:RHEA-COMP:15547, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:16842, ChEBI:CHEBI:17499, ChEBI:CHEBI:29969, ChEBI:CHEBI:61976; EC=1.14.99.66; Evidence={ECO:0000269|PubMed:23260659, ECO:0000269|PubMed:23357850}; |
| Cofactor: | |
Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000269|PubMed:23260659, ECO:0000269|PubMed:23266887, ECO:0000269|PubMed:23357850}; |
| Cofactor: | |
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:23260659, ECO:0000269|PubMed:23266887, ECO:0000269|PubMed:23357850}; Note=Binds 3 Zn(2+) ions per subunit. {ECO:0000269|PubMed:23260659, ECO:0000269|PubMed:23266887, ECO:0000269|PubMed:23357850}; |
| Activity regulation: | |
Histone H3K4me1 and H3K4me2 demethylase activity is enhanced by GLYR1. {ECO:0000269|PubMed:23260659}. |
| Subunit: | |
Does not form a complex with RCOR1/CoREST (By similarity). Interacts with its cofactor GLYR1 at nucleosomes; this interaction stimulates H3K4me1 and H3K4me2 demethylation (PubMed:23260659). {ECO:0000250, ECO:0000269|PubMed:23260659}. |
| Subcellular location: | |
Nucleus {ECO:0000250}. |
| Domain: | |
The SWIRM domain may act as an anchor site for a histone tail. {ECO:0000250}. |
| Similarity: | |
Belongs to the flavin monoamine oxidase family. {ECO:0000305}. |
| Sequence caution: | |
Sequence=BAC03663.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.