PDBsum entry 4gcz

Signaling protein, de novo protein

PDB id: 4gcz

Contents

Protein chains

366 a.a.

Ligands

FMN ×2

ADP

SO4 ×10

Waters ×451

References listed in PDB file

Key reference

• Title: Full-Length structure of a sensor histidine kinase pinpoints coaxial coiled coils as signal transducers and modulators.
• Authors: R.P.Diensthuber, M.Bommer, T.Gleichmann, A.Möglich.
• Ref.: Structure, 2013, 21, 1127-1136. [DOI no: 10.1016/j.str.2013.04.024]
• PubMed id: 23746806

Abstract

Two-component systems (TCSs), which comprise sensor histidine kinases (SHK) and response-regulator proteins, represent the predominant strategy by which prokaryotes sense and respond to a changing environment. Despite paramount biological importance, a dearth exists of intact SHK structures containing both sensor and effector modules. Here, we report the full-length crystal structure of the engineered, dimeric, blue-light-regulated SHK YF1 at 2.3 Å resolution, in which two N-terminal light-oxygen-voltage (LOV) photosensors are connected by a coiled coil to the C-terminal effector modules. A second coaxial coiled coil derived from the N-termini of the LOV photosensors and inserted between them crucially modulates light regulation: single mutations within this coiled coil attenuate or even invert the signal response of the TCS. Structural motifs identified in YF1 recur in signal receptors, and the underlying signaling principles and mechanisms may be widely shared between soluble and transmembrane, prokaryotic, and eukaryotic signal receptors of diverse biological activity.