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PDBsum entry 4g8e
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Immune system
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PDB id
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4g8e
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References listed in PDB file
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Key reference
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Title
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A conserved human t cell population targets mycobacterial antigens presented by cd1b.
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Authors
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I.Van rhijn,
A.Kasmar,
A.De jong,
S.Gras,
M.Bhati,
M.E.Doorenspleet,
N.De vries,
D.I.Godfrey,
J.D.Altman,
W.De jager,
J.Rossjohn,
D.B.Moody.
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Ref.
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Nat Immunol, 2013,
14,
706-713.
[DOI no: ]
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PubMed id
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Abstract
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Human T cell antigen receptors (TCRs) pair in millions of combinations to create
complex and unique T cell repertoires for each person. Through the use of
tetramers to analyze TCRs reactive to the antigen-presenting molecule CD1b, we
detected T cells with highly stereotyped TCR α-chains present among genetically
unrelated patients with tuberculosis. The germline-encoded, mycolyl
lipid-reactive (GEM) TCRs had an α-chain bearing the variable (V) region
TRAV1-2 rearranged to the joining (J) region TRAJ9 with few nontemplated
(N)-region additions. Analysis of TCRs by high-throughput sequencing, binding
and crystallography showed linkage of TCRα sequence motifs to high-affinity
recognition of antigen. Thus, the CD1-reactive TCR repertoire is composed of at
least two compartments: high-affinity GEM TCRs, and more-diverse TCRs with low
affinity for CD1b-lipid complexes. We found high interdonor conservation of TCRs
that probably resulted from selection by a nonpolymorphic antigen-presenting
molecule and an immunodominant antigen.
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