PDBsum entry 4g8a

Immune system

PDB id: 4g8a

Contents

Protein chains

601 a.a.

140 a.a.

Ligands

NAG-NAG ×4

NAG ×4

LP4-LP5-DAO-MYR-KDO ×2

Waters ×39

PDB id:

4g8a

Name:

Immune system

Title:

Crystal structure of human tlr4 polymorphic variant d299g and t399i in complex with md-2 and lps

Structure:

Toll-like receptor 4. Chain: a, b. Fragment: unp residues 23-629. Synonym: htoll, protein tlr4. Engineered: yes. Mutation: yes. Lymphocyte antigen 96. Chain: c, d. Synonym: ly-96, esop-1, protein md-2.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: tlr4. Expressed in: drosophila. Expression_system_taxid: 7215. Expression_system_cell_line: s2. Gene: ly96, esop1, md2.

Resolution:

2.40Å R-factor: 0.202 R-free: 0.249

Authors:

U.Ohto,T.Shimizu

Key ref:

U.Ohto et al. (2012). Structural analyses of human Toll-like receptor 4 polymorphisms D299G and T399I. J Biol Chem, 287, 40611-40617. PubMed id: 23055527

Date:

23-Jul-12 Release date: 17-Oct-12

Protein chains

O00206  (TLR4_HUMAN) -  Toll-like receptor 4 from Homo sapiens

Seq: 839 a.a.

Struc: 601 a.a.*

Protein chains

Q9Y6Y9  (LY96_HUMAN) -  Lymphocyte antigen 96 from Homo sapiens

Seq: 160 a.a.

Struc: 140 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B: E.C.3.2.2.6 - ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase.
• Reaction: NAD⁺ + H2O = ADP-D-ribose + nicotinamide + H⁺

NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+) (Bound ligand (Het Group name = NAG) matches with 43.75% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

J Biol Chem 287:40611-40617 (2012)

PubMed id: 23055527

Structural analyses of human Toll-like receptor 4 polymorphisms D299G and T399I.

U.Ohto, N.Yamakawa, S.Akashi-Takamura, K.Miyake, T.Shimizu.

ABSTRACT

No abstract given.