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PDBsum entry 4g7z
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Transcription, transferase/DNA
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PDB id
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4g7z
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Contents |
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226 a.a.
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1111 a.a.
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1482 a.a.
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94 a.a.
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346 a.a.
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References listed in PDB file
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Key reference
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Title
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Structural basis of transcription initiation.
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Authors
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Y.Zhang,
Y.Feng,
S.Chatterjee,
S.Tuske,
M.X.Ho,
E.Arnold,
R.H.Ebright.
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Ref.
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Science, 2012,
338,
1076-1080.
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PubMed id
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Abstract
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During transcription initiation, RNA polymerase (RNAP) binds and unwinds
promoter DNA to form an RNAP-promoter open complex. We have determined crystal
structures at 2.9 and 3.0 Å resolution of functional transcription initiation
complexes comprising Thermus thermophilus RNA polymerase, σ(A), and a promoter
DNA fragment corresponding to the transcription bubble and downstream
double-stranded DNA of the RNAP-promoter open complex. The structures show that
σ recognizes the -10 element and discriminator element through interactions
that include the unstacking and insertion into pockets of three DNA bases and
that RNAP recognizes the -4/+2 region through interactions that include the
unstacking and insertion into a pocket of the +2 base. The structures further
show that interactions between σ and template-strand single-stranded DNA
(ssDNA) preorganize template-strand ssDNA to engage the RNAP active center.
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