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PDBsum entry 4fin
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Atp-binding protein
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PDB id
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4fin
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References listed in PDB file
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Key reference
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Title
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The abc-F protein etta gates ribosome entry into the translation elongation cycle.
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Authors
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G.Boël,
P.C.Smith,
W.Ning,
M.T.Englander,
B.Chen,
Y.Hashem,
A.J.Testa,
J.J.Fischer,
H.J.Wieden,
J.Frank,
R.L.Gonzalez,
J.F.Hunt.
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Ref.
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Nat Struct Biol, 2014,
21,
143-151.
[DOI no: ]
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PubMed id
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Abstract
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ABC-F proteins have evaded functional characterization even though they compose
one of the most widely distributed branches of the ATP-binding cassette (ABC)
superfamily. Herein, we demonstrate that YjjK, the most prevalent eubacterial
ABC-F protein, gates ribosome entry into the translation elongation cycle
through a nucleotide-dependent interaction sensitive to ATP/ADP ratio.
Accordingly, we rename this protein energy-dependent translational throttle A
(EttA). We determined the crystal structure of Escherichia coli EttA and used it
to design mutants for biochemical studies including enzymological assays of the
initial steps of protein synthesis. These studies suggest that EttA may regulate
protein synthesis in energy-depleted cells, which have a low ATP/ADP ratio.
Consistently with this inference, EttA-deleted cells exhibit a severe fitness
defect in long-term stationary phase. These studies demonstrate that an ABC-F
protein regulates protein synthesis via a new mechanism sensitive to cellular
energy status.
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