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PDBsum entry 4fdg
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References listed in PDB file
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Key reference
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Title
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Mini-Chromosome maintenance complexes form a filament to remodel DNA structure and topology.
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Authors
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I.M.Slaymaker,
Y.Fu,
D.B.Toso,
N.Ranatunga,
A.Brewster,
S.L.Forsburg,
Z.H.Zhou,
X.S.Chen.
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Ref.
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Nucleic Acids Res, 2013,
41,
3446-3456.
[DOI no: ]
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PubMed id
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Abstract
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Deregulation of mini-chromosome maintenance (MCM) proteins is associated with
genomic instability and cancer. MCM complexes are recruited to replication
origins for genome duplication. Paradoxically, MCM proteins are in excess than
the number of origins and are associated with chromatin regions away from the
origins during G1 and S phases. Here, we report an unusually wide left-handed
filament structure for an archaeal MCM, as determined by X-ray and electron
microscopy. The crystal structure reveals that an α-helix bundle formed between
two neighboring subunits plays a critical role in filament formation. The
filament has a remarkably strong electro-positive surface spiraling along the
inner filament channel for DNA binding. We show that this MCM filament binding
to DNA causes dramatic DNA topology change. This newly identified function of
MCM to change DNA topology may imply a wider functional role for MCM in DNA
metabolisms beyond helicase function. Finally, using yeast genetics, we show
that the inter-subunit interactions, important for MCM filament formation, play
a role for cell growth and survival.
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