PDBsum entry 4fdg

Hydrolase

PDB id: 4fdg

Contents

Protein chains

592 a.a.

Metals

_ZN ×5

PDB id:

4fdg

Name:

Hydrolase

Title:

Crystal structure of an archaeal mcm filament

Structure:

Minichromosome maintenance protein mcm. Chain: b, a, c, d, e. Fragment: unp residues 7-686. Engineered: yes

Source:

Sulfolobus solfataricus. Organism_taxid: 2287. Strain: p2. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

4.10Å R-factor: 0.332 R-free: 0.341

Authors:

I.M.Slaymaker,Y.Fu,A.B.Brewster,X.S.Chen

Key ref:

I.M.Slaymaker et al. (2013). Mini-chromosome maintenance complexes form a filament to remodel DNA structure and topology. Nucleic Acids Res, 41, 3446-3456. PubMed id: 23361460 DOI: 10.1093/nar/gkt022

Date:

28-May-12 Release date: 06-Mar-13

Protein chains

Q9UXG1  (MCM_SULSO) -  Minichromosome maintenance protein MCM from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Seq: 686 a.a.

Struc: 592 a.a.

Enzyme reactions

• Enzyme class: E.C.3.6.4.12 - Dna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1093/nar/gkt022

Nucleic Acids Res 41:3446-3456 (2013)

PubMed id: 23361460

Mini-chromosome maintenance complexes form a filament to remodel DNA structure and topology.

I.M.Slaymaker, Y.Fu, D.B.Toso, N.Ranatunga, A.Brewster, S.L.Forsburg, Z.H.Zhou, X.S.Chen.

ABSTRACT

Deregulation of mini-chromosome maintenance (MCM) proteins is associated with genomic instability and cancer. MCM complexes are recruited to replication origins for genome duplication. Paradoxically, MCM proteins are in excess than the number of origins and are associated with chromatin regions away from the origins during G1 and S phases. Here, we report an unusually wide left-handed filament structure for an archaeal MCM, as determined by X-ray and electron microscopy. The crystal structure reveals that an α-helix bundle formed between two neighboring subunits plays a critical role in filament formation. The filament has a remarkably strong electro-positive surface spiraling along the inner filament channel for DNA binding. We show that this MCM filament binding to DNA causes dramatic DNA topology change. This newly identified function of MCM to change DNA topology may imply a wider functional role for MCM in DNA metabolisms beyond helicase function. Finally, using yeast genetics, we show that the inter-subunit interactions, important for MCM filament formation, play a role for cell growth and survival.