PDBsum entry 4fbj

Cell cycle/protein binding

PDB id: 4fbj

Contents

Protein chains

250 a.a.

74 a.a.

Metals

_CL

Waters ×376

PDB id:

4fbj

Name:

Cell cycle/protein binding

Title:

Structure of the cif:nedd8 complex - photorhabdus luminescens cycle inhibiting factor in complex with human nedd8

Structure:

Hypothetical protein. Chain: a. Fragment: effector domain, unp residues 53-313. Synonym: cif. Engineered: yes. Mutation: yes. Nedd8. Chain: b. Synonym: neddylin, neural precursor cell expressed developmentally

Source:

Photorhabdus luminescens subsp. Laumondii. Organism_taxid: 243265. Strain: tt01. Gene: plu2515. Expressed in: escherichia coli. Expression_system_taxid: 562. Homo sapiens. Human. Organism_taxid: 9606.

Resolution:

1.60Å R-factor: 0.168 R-free: 0.236

Authors:

A.Crow,M.J.Banfield

Key ref:

A.Crow et al. The molecular basis of nedd8 deamidation by the bacte effector protein cif. Proc natl acad sci usa, .

Date:

23-May-12 Release date: 20-Jun-12

Protein chain

Q7N439  (Q7N439_PHOLL) -  Protein-glutamine deamidase Cif from Photorhabdus laumondii subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01)

Seq: 313 a.a.

Struc: 250 a.a.*

Protein chain

Q15843  (NEDD8_HUMAN) -  NEDD8 from Homo sapiens

Seq: 81 a.a.

Struc: 74 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.5.1.44 - protein-glutamine glutaminase.
• Reaction: L-glutaminyl-[protein] + H2O = L-glutamyl-[protein] + NH4⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site