PDBsum entry 4f6m

DNA binding protein/DNA

PDB id: 4f6m

Contents

Protein chain

116 a.a.

DNA/RNA

Metals

_ZN ×3

Waters ×83

PDB id:

4f6m

Name:

DNA binding protein/DNA

Title:

Crystal structure of kaiso zinc finger DNA binding domain in complex with kaiso binding site DNA

Structure:

Transcriptional regulator kaiso. Chain: a. Fragment: zinc finger DNA binding domain. Synonym: zinc finger and btb domain-containing protein 33. Engineered: yes. DNA (5'- d( Gp Tp Gp Cp Tp Tp Cp Cp Tp Gp Cp Cp Ap Ap Tp Ap Ap Cp G)-3'). Chain: d. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: zbtb33, kaiso, znf348. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Other_details: this sequence occurs in humans. Other_details: this sequence occurs in humans

Resolution:

2.40Å R-factor: 0.223 R-free: 0.240

Authors:

B.A.Buck-Koehntop,R.L.Stanfield,D.C.Ekiert,M.A.Martinez-Yamout, H.J.Dyson,I.A.Wilson,P.E.Wright

Key ref:

B.A.Buck-Koehntop et al. (2012). Molecular basis for recognition of methylated and specific DNA sequences by the zinc finger protein Kaiso. Proc Natl Acad Sci U S A, 109, 15229-15234. PubMed id: 22949637

Date:

15-May-12 Release date: 05-Sep-12

Protein chain

Q86T24  (KAISO_HUMAN) -  Transcriptional regulator Kaiso from Homo sapiens

Seq: 672 a.a.

Struc: 116 a.a.

DNA/RNA chains

G-T-G-C-T-T-C-C-T-G-C-C-A-A-T-A-A-C-G 19 bases

C-G-T-T-A-T-T-G-G-C-A-G-G-A-A-G-C-A-C 19 bases

Enzyme reactions

• Enzyme class: E.C.?

Proc Natl Acad Sci U S A 109:15229-15234 (2012)

PubMed id: 22949637

Molecular basis for recognition of methylated and specific DNA sequences by the zinc finger protein Kaiso.

B.A.Buck-Koehntop, R.L.Stanfield, D.C.Ekiert, M.A.Martinez-Yamout, H.J.Dyson, I.A.Wilson, P.E.Wright.

ABSTRACT

No abstract given.