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UniProt functional annotation for P24215 | ||
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| UniProt code: P24215. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Catalyzes the dehydration of D-mannonate. {ECO:0000269|PubMed:3038546}. |
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| Catalytic activity: | |
Reaction=D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H2O; Xref=Rhea:RHEA:20097, ChEBI:CHEBI:15377, ChEBI:CHEBI:17767, ChEBI:CHEBI:57990; EC=4.2.1.8; Evidence={ECO:0000269|PubMed:3038546}; |
| Cofactor: | |
Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000269|PubMed:3038546}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:3038546}; Note=Mn(2+) can substitute for iron, but in higher concentrations. Cannot use Fe(3+), Ni(2+) or Mg(2+). {ECO:0000269|PubMed:3038546}; |
| Activity regulation: | |
Is inhibited by high concentrations of Fe(2+) (> 2 mM), and by EDTA or other iron chelators in vitro. {ECO:0000269|PubMed:3038546}. |
| Biophysicochemical properties: | |
pH dependence: Optimum pH is about 7.5. The activity at pH 6.5 and 8.6 is only about 20% of the maximal achievable activity at optimum pH. {ECO:0000269|PubMed:3038546}; |
| Pathway: | |
Carbohydrate metabolism; pentose and glucuronate interconversion. |
| Induction: | |
By fructuronate. Its expression is subjected to catabolite repression by glucose. |
| Similarity: | |
Belongs to the mannonate dehydratase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.