PDBsum entry 4e5z

DNA binding protein/DNA

PDB id: 4e5z

Contents

Protein chains

1140 a.a.

402 a.a.

DNA/RNA

PDB id:

4e5z

Name:

DNA binding protein/DNA

Title:

Damaged DNA induced uv-damaged DNA-binding protein (uv-ddb) dimerization and its roles in chromatinized DNA repair

Structure:

DNA damage-binding protein 1. Chain: a. Synonym: ddb p127 subunit, DNA damage-binding protein a, ddba, damage-specific DNA-binding protein 1, hbv x-associated protein 1, xap-1, uv-damaged DNA-binding factor, uv-damaged DNA-binding protein 1, uv-ddb 1, xpe-binding factor, xpe-bf, xeroderma pigmentosum group e-complementing protein, xpce. Engineered: yes. DNA damage-binding protein 2.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ddb1, ddb1_human, q16531, xap1. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9. Ddb1pbluebac4.5/v5-his nt-his10-ddb1. Gene: ddb2.

Resolution:

3.22Å R-factor: 0.230 R-free: 0.284

Authors:

J.I.Yeh,S.Du

Key ref:

J.I.Yeh et al. (2012). Damaged DNA induced UV-damaged DNA-binding protein (UV-DDB) dimerization and its roles in chromatinized DNA repair. Proc Natl Acad Sci U S A, 109, E2737. PubMed id: 22822215

Date:

15-Mar-12 Release date: 22-Aug-12

Protein chain

Q16531  (DDB1_HUMAN) -  DNA damage-binding protein 1 from Homo sapiens

Seq: 1140 a.a.

Struc: 1140 a.a.*

Protein chain

Q92466  (DDB2_HUMAN) -  DNA damage-binding protein 2 from Homo sapiens

Seq: 427 a.a.

Struc: 402 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

DNA/RNA chains

T-G-A-C-T-G-T-A-T-G-A-T-G-A-C-G-A-T-G-C-T-G-A-C 24 bases

G-T-C-A-G-C-A-T-C-G-3DR-C-A-T-C-A-T-A-C-A-G-T-C-A 24 bases

Proc Natl Acad Sci U S A 109:E2737 (2012)

PubMed id: 22822215

Damaged DNA induced UV-damaged DNA-binding protein (UV-DDB) dimerization and its roles in chromatinized DNA repair.

J.I.Yeh, A.S.Levine, S.Du, U.Chinte, H.Ghodke, H.Wang, H.Shi, C.L.Hsieh, J.F.Conway, B.Van Houten, V.Rapić-Otrin.

ABSTRACT

No abstract given.