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PDBsum entry 4e1g
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Oxidoreductase
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PDB id
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4e1g
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PDB id:
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| Name: |
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Oxidoreductase
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Title:
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X-ray crystal structure of alpha-linolenic acid bound to the cyclooxygenase channel of cyclooxygenase-2
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Structure:
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Prostaglandin g/h synthase 2. Chain: a, b. Fragment: unp residues 1-604. Synonym: cyclooxygenase-2, cox-2, glucocorticoid-regulated inflammatory cyclooxygenase, gripghs, macrophage activation- associated marker protein p71/73, pes-2, phs ii, prostaglandin h2 synthase 2, pgh synthase 2, pghs-2, prostaglandin-endoperoxide synthase 2, tis10 protein. Engineered: yes.
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Source:
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Mus musculus. Mouse. Organism_taxid: 10090. Strain: wild type. Gene: cox-2, cox2, pghs-b, ptgs2, tis10. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108.
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Resolution:
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2.10Å
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R-factor:
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0.161
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R-free:
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0.203
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Authors:
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A.J.Vecchio,M.G.Malkowski
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Key ref:
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A.J.Vecchio
et al.
(2012).
Investigating substrate promiscuity in cyclooxygenase-2: the role of Arg-120 and residues lining the hydrophobic groove.
J Biol Chem,
287,
24619-24630.
PubMed id:
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Date:
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06-Mar-12
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Release date:
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25-Apr-12
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PROCHECK
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Headers
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References
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Q05769
(PGH2_MOUSE) -
Prostaglandin G/H synthase 2 from Mus musculus
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Seq:
Struc:
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604 a.a.
551 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 2 residue positions (black
crosses)
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Enzyme class:
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E.C.1.14.99.1
- prostaglandin-endoperoxide synthase.
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Reaction:
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(5Z,8Z,11Z,14Z)-eicosatetraenoate + AH2 + 2 O2 = prostaglandin H2 + A + H2O
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(5Z,8Z,11Z,14Z)-eicosatetraenoate
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+
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AH2
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+
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2
×
O2
Bound ligand (Het Group name = )
matches with 50.00% similarity
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=
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prostaglandin H2
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+
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+
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H2O
Bound ligand (Het Group name = )
matches with 51.11% similarity
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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J Biol Chem
287:24619-24630
(2012)
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PubMed id:
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Investigating substrate promiscuity in cyclooxygenase-2: the role of Arg-120 and residues lining the hydrophobic groove.
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A.J.Vecchio,
B.J.Orlando,
R.Nandagiri,
M.G.Malkowski.
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ABSTRACT
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Links
