PDBsum entry 4drx

Cell cycle

PDB id: 4drx

Contents

Protein chains

431 a.a.

424 a.a.

156 a.a.

Ligands

GTP ×4

Metals

_MG ×4

Waters ×345

PDB id:

4drx

Name:

Cell cycle

Title:

Gtp-tubulin in complex with a darpin

Structure:

Tubulin alpha chain. Chain: a, c. Fragment: unp residues 1-437. Tubulin beta chain. Chain: b, d. Fragment: unp residues 1-431. Designed ankyrin repeat protein (darpin) d1. Chain: e, f. Engineered: yes

Source:

Ovis aries. Domestic sheep,lambs,wild sheep. Organism_taxid: 9940. Artificial gene. Organism_taxid: 32630. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.22Å R-factor: 0.161 R-free: 0.194

Authors:

L.Pecqueur,B.Gigant,M.Knossow

Key ref:

L.Pecqueur et al. (2012). A designed ankyrin repeat protein selected to bind to tubulin caps the microtubule plus end. Proc Natl Acad Sci U S A, 109, 12011-12016. PubMed id: 22778434

Date:

17-Feb-12 Release date: 11-Jul-12

Protein chains

D0VWZ0  (D0VWZ0_SHEEP) -  Tubulin alpha chain from Ovis aries

Seq: 451 a.a.

Struc: 431 a.a.*

Protein chains

D0VWY9  (D0VWY9_SHEEP) -  Tubulin beta chain from Ovis aries

Seq: 445 a.a.

Struc: 424 a.a.*

Protein chains

No UniProt id for this chain

Struc: 156 a.a.

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Proc Natl Acad Sci U S A 109:12011-12016 (2012)

PubMed id: 22778434

A designed ankyrin repeat protein selected to bind to tubulin caps the microtubule plus end.

L.Pecqueur, C.Duellberg, B.Dreier, Q.Jiang, C.Wang, A.Plückthun, T.Surrey, B.Gigant, M.Knossow.

ABSTRACT

No abstract given.