PDBsum entry 4djz

Hydrolase/hydrolase inhibitor

PDB id: 4djz

Contents

Protein chains

148 a.a.

251 a.a.

34 a.a.

33 a.a.

Waters ×2

PDB id:

4djz

Name:

Hydrolase/hydrolase inhibitor

Title:

Catalytic fragment of masp-1 in complex with its specific inhibitor developed by directed evolution on sgci scaffold

Structure:

Mannan-binding lectin serine protease 1 heavy chain. Chain: a, c. Fragment: sushi domain residues 298-448. Synonym: mannan-binding lectin serine protease 1, complement factor masp-3, complement-activating component of ra-reactive factor, mannose-binding lectin-associated serine protease 1, masp-1, mannose- binding protein-associated serine protease, ra-reactive factor serine protease p100, rarf, serine protease 5. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: crarf, crarf1, masp1, prss5. Expressed in: escherichia coli. Expression_system_taxid: 469008. Schistocerca gregaria. Desert locust. Organism_taxid: 7010.

Resolution:

3.20Å R-factor: 0.230 R-free: 0.274

Authors:

D.Heja,V.Harmat,K.Fodor,M.Wilmanns,J.Dobo,K.A.Kekesi,P.Zavodszky, P.Gal,G.Pal

Key ref:

D.Héja et al. (2012). Monospecific inhibitors show that both mannan-binding lectin-associated serine protease-1 (MASP-1) and -2 Are essential for lectin pathway activation and reveal structural plasticity of MASP-2. J Biol Chem, 287, 20290-20300. PubMed id: 22511776

Date:

03-Feb-12 Release date: 25-Apr-12

Protein chains

P48740  (MASP1_HUMAN) -  Mannan-binding lectin serine protease 1 from Homo sapiens

Seq: 699 a.a.

Struc: 148 a.a.*

Protein chains

P48740  (MASP1_HUMAN) -  Mannan-binding lectin serine protease 1 from Homo sapiens

Seq: 699 a.a.

Struc: 251 a.a.*

Protein chain

O46162  (SGP1_SCHGR) -  Serine protease inhibitor I/II from Schistocerca gregaria

Seq: 92 a.a.

Struc: 34 a.a.*

Protein chain

O46162  (SGP1_SCHGR) -  Serine protease inhibitor I/II from Schistocerca gregaria

Seq: 92 a.a.

Struc: 33 a.a.*

* PDB and UniProt seqs differ at 10 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B, C, D: E.C.3.4.21.- - ?????

J Biol Chem 287:20290-20300 (2012)

PubMed id: 22511776

Monospecific inhibitors show that both mannan-binding lectin-associated serine protease-1 (MASP-1) and -2 Are essential for lectin pathway activation and reveal structural plasticity of MASP-2.

D.Héja, V.Harmat, K.Fodor, M.Wilmanns, J.Dobó, K.A.Kékesi, P.Závodszky, P.Gál, G.Pál.

ABSTRACT

No abstract given.