PDBsum entry 4dih

Hydrolase/hydrolase inhibitor/DNA

PDB id: 4dih

Contents

Protein chains

27 a.a.

249 a.a.

DNA/RNA

Ligands

0G6

NAG

_NA ×2

Metals

_ZN ×3

_CL ×2

Waters ×217

PDB id:

4dih

Name:

Hydrolase/hydrolase inhibitor/DNA

Title:

X-ray structure of the complex between human alpha thrombin and thrombin binding aptamer in the presence of sodium ions

Structure:

Prothrombin. Chain: l. Fragment: light chain fragment (unp residues 328-363). Synonym: coagulation factor ii, thrombin light chain. Prothrombin. Chain: h. Fragment: heavy chain fragment (unp residues 364-622). Synonym: coagulation factor ii, thrombin heavy chain. Thrombin binding aptamer.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Synthetic: yes. Synthetic DNA. Organism_taxid: 32630

Resolution:

1.80Å R-factor: 0.167 R-free: 0.213

Authors:

I.Russo Krauss,A.Merlino,L.Mazzarella,F.Sica

Key ref:

I.Russo Krauss et al. (2012). High-resolution structures of two complexes between thrombin and thrombin-binding aptamer shed light on the role of cations in the aptamer inhibitory activity. Nucleic Acids Res, 40, 8119-8128. PubMed id: 22669903

Date:

31-Jan-12 Release date: 18-Jul-12

Protein chain

P00734  (THRB_HUMAN) -  Prothrombin from Homo sapiens

Seq: 622 a.a.

Struc: 27 a.a.

Protein chain

P00734  (THRB_HUMAN) -  Prothrombin from Homo sapiens

Seq: 622 a.a.

Struc: 249 a.a.

DNA/RNA chain

G-G-T-T-G-G-T-G-T-G-G-T-T-G-G 15 bases

Enzyme reactions

• Enzyme class: Chains L, H: E.C.3.4.21.5 - thrombin.
• Reaction: Preferential cleavage: Arg-|-Gly; activates fibrinogen to fibrin and releases fibrinopeptide A and B.

Nucleic Acids Res 40:8119-8128 (2012)

PubMed id: 22669903

High-resolution structures of two complexes between thrombin and thrombin-binding aptamer shed light on the role of cations in the aptamer inhibitory activity.

I.Russo Krauss, A.Merlino, A.Randazzo, E.Novellino, L.Mazzarella, F.Sica.

ABSTRACT

No abstract given.