 |
PDBsum entry 4dhj
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Hydrolase/signaling protein/ligase
|
PDB id
|
|
|
|
4dhj
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
|
|
|
248 a.a.
|
|
|
|
|
|
|
|
|
|
|
76 a.a.
|
|
|
|
|
|
|
|
|
|
|
71 a.a.
|
|
|
|
|
|
|
|
|
|
|
149 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
|
PDB id:
|
|
|
|
| Name: |
|
Hydrolase/signaling protein/ligase
|
|
|
Title:
|
|
The structure of a ceotub1 ubiquitin aldehyde ubc13~ub complex
|
|
Structure:
|
|
Ubiquitin thioesterase otubain-like. Chain: a, e, i, l. Synonym: otub1, deubiquitinating enzyme otubain-like, ubiquitin- specific-processing protease otubain-like. Engineered: yes. Ubiquitin aldehyde. Chain: b, f, j, m. Engineered: yes. Ubiquitin.
|
|
Source:
|
|
Caenorhabditis elegans. Nematode. Organism_taxid: 6239. Gene: otub-1, c25d7.8. Expressed in: escherichia coli. Expression_system_taxid: 562. Homo sapiens. Human. Organism_taxid: 9606.
|
|
Resolution:
|
|
|
2.35Å
|
R-factor:
|
0.204
|
R-free:
|
0.237
|
|
|
Authors:
|
|
R.Wiener,X.Zhang,T.Wang,C.Wolberger
|
|
Key ref:
|
|
R.Wiener
et al.
(2012).
The mechanism of OTUB1-mediated inhibition of ubiquitination.
Nature,
483,
618-622.
PubMed id:
|
|
|
Date:
|
|
|
27-Jan-12
|
Release date:
|
22-Feb-12
|
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
|
Headers
|
|
|
|
References
|
|
|
|
|
|
|
|
Q9XVR6
(OTUBL_CAEEL) -
Ubiquitin thioesterase otubain-like from Caenorhabditis elegans
|
|
|
|
Seq:
Struc:
|
|
|
|
284 a.a.
248 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
P0CG48
(UBC_HUMAN) -
Polyubiquitin-C from Homo sapiens
|
|
|
|
Seq:
Struc:
|
|
|
|
685 a.a.
76 a.a.*
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Enzyme class 2:
|
|
Chains A, E, I, L:
E.C.3.4.19.12
- ubiquitinyl hydrolase 1.
|
|
|
|
|
|
|
Reaction:
|
|
Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
|
|
|
|
|
|
Enzyme class 3:
|
|
Chains C, K, G, N:
E.C.2.3.2.23
- E2 ubiquitin-conjugating enzyme.
|
|
|
|
|
|
|
Reaction:
|
|
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine
|
|
|
|
|
|
|
|
|
Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
| |
|
|
Nature
483:618-622
(2012)
|
|
PubMed id:
|
|
|
|
|
|
| |
|
The mechanism of OTUB1-mediated inhibition of ubiquitination.
|
|
R.Wiener,
X.Zhang,
T.Wang,
C.Wolberger.
|
|
|
|
|
| |
ABSTRACT
|
|
|
|
| |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
|
Literature references that cite this PDB file's key reference
|
|
|
| |
PubMed id
|
|
Reference
|
|
|
|
|
|
Y.Ye,
G.Blaser,
M.H.Horrocks,
M.J.Ruedas-Rama,
S.Ibrahim,
A.A.Zhukov,
A.Orte,
D.Klenerman,
S.E.Jackson,
and
D.Komander
(2012).
Ubiquitin chain conformation regulates recognition and activity of interacting proteins.
|
| |
Nature,
492,
266-270.
|
|
|
|
|
|
The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
|
| | |
Links
