PDBsum entry 4dbc

Transferase

PDB id: 4dbc

Contents

Protein chain

396 a.a.

Ligands

SO4 ×4

EDO

3QP

Waters ×447

References listed in PDB file

Key reference

• Title: Ground-State electronic destabilization via hyperconjugation in aspartate aminotransferase.
• Authors: W.R.Griswold, J.N.Castro, A.J.Fisher, M.D.Toney.
• Ref.: J Am Chem Soc, 2012, 134, 8436-8438.
• PubMed id: 22551424

Abstract

Binding isotope effects for l-aspartate reacting with the inactive K258A mutant of PLP-dependent aspartate aminotransferase to give a stable external aldimine intermediate are reported. They provide direct evidence for electronic ground-state destabilization via hyperconjugation. The smaller equilibrium isotope effect with deazaPLP-reconstituted K258A indicates that the pyridine nitrogen plays an important role in labilizing the Cα-H bond.