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PDBsum entry 4d6v
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Signaling protein
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PDB id
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4d6v
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PDB id:
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| Name: |
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Signaling protein
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Title:
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Crystal structure of signal transducing protein
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Structure:
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G protein beta subunit gib2. Chain: a. Synonym: gib2. Engineered: yes
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Source:
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Cryptococcus neoformans var. Grubii h99. Organism_taxid: 235443. Strain: h99. Expressed in: escherichia coli. Expression_system_taxid: 511693.
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Resolution:
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2.20Å
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R-factor:
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0.197
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R-free:
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0.231
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Authors:
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R.Ero,V.T.Dimitrova,Y.Chen,W.Bu,S.Feng,T.Liu,P.Wang,C.Xue,S.M.Tan, Y.G.Gao
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Key ref:
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R.Ero
et al.
(2015).
Crystal structure of Gib2, a signal-transducing protein scaffold associated with ribosomes in Cryptococcus neoformans.
Sci Rep,
5,
8688.
PubMed id:
DOI:
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Date:
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17-Nov-14
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Release date:
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18-Mar-15
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PROCHECK
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Headers
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References
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J9W3X8
(J9W3X8_CRYNH) -
Guanine nucleotide-binding protein subunit beta-like protein from Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
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Seq:
Struc:
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314 a.a.
313 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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DOI no:
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Sci Rep
5:8688
(2015)
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PubMed id:
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Crystal structure of Gib2, a signal-transducing protein scaffold associated with ribosomes in Cryptococcus neoformans.
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R.Ero,
V.T.Dimitrova,
Y.Chen,
W.Bu,
S.Feng,
T.Liu,
P.Wang,
C.Xue,
S.M.Tan,
Y.G.Gao.
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ABSTRACT
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The atypical Gβ-like/RACK1 Gib2 protein promotes cAMP signalling that plays a
central role in regulating the virulence of Cryptococcus neoformans. Gib2
contains a seven-bladed β transducin structure and is emerging as a scaffold
protein interconnecting signalling pathways through interactions with various
protein partners. Here, we present the crystal structure of Gib2 at a 2.2-Å
resolution. The structure allows us to analyse the association between Gib2 and
the ribosome, as well as to identify the Gib2 amino acid residues involved in
ribosome binding. Our studies not only suggest that Gib2 has a role in protein
translation but also present Gib2 as a physical link at the crossroads of
various regulatory pathways important for the growth and virulence of C.
neoformans.
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Links
