PDBsum entry 4ct4

RNA binding protein

PDB id: 4ct4

Contents

Protein chains

236 a.a.

378 a.a.

Metals

_MG ×4

_CL

Waters ×268

PDB id:

4ct4

Name:

RNA binding protein

Title:

Cnot1 mif4g domain - ddx6 complex

Structure:

Ccr4-not transcription complex subunit 1. Chain: a, c. Fragment: mif4g domain, residues 1063-1314. Synonym: ccr4-associated factor 1, negative regulator of transcription subunit 1 homolog, not1h, hnot1. Engineered: yes. Probable atp-dependent RNA helicase ddx6. Chain: b, d. Fragment: reca1 and reca2, residues 95-469.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: prare.

Resolution:

2.30Å R-factor: 0.169 R-free: 0.220

Authors:

S.Ozgur,J.Basquin,E.Conti

Key ref:

H.Mathys et al. (2014). Structural and biochemical insights to the role of the CCR4-NOT complex and DDX6 ATPase in microRNA repression. Mol Cell, 54, 751-765. PubMed id: 24768538 DOI: 10.1016/j.molcel.2014.03.036

Date:

12-Mar-14 Release date: 07-May-14

Protein chains

A5YKK6  (CNOT1_HUMAN) -  CCR4-NOT transcription complex subunit 1 from Homo sapiens

Seq: 2376 a.a.

Struc: 236 a.a.

Protein chains

P26196  (DDX6_HUMAN) -  Probable ATP-dependent RNA helicase DDX6 from Homo sapiens

Seq: 483 a.a.

Struc: 378 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains B, D: E.C.3.6.4.13 - Rna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1016/j.molcel.2014.03.036

Mol Cell 54:751-765 (2014)

PubMed id: 24768538

Structural and biochemical insights to the role of the CCR4-NOT complex and DDX6 ATPase in microRNA repression.

H.Mathys, J.Basquin, S.Ozgur, M.Czarnocki-Cieciura, F.Bonneau, A.Aartse, A.Dziembowski, M.Nowotny, E.Conti, W.Filipowicz.

ABSTRACT

No abstract given.