PDBsum entry 4crw

Gene regulation

PDB id: 4crw

Contents

Protein chains

226 a.a.

158 a.a.

Ligands

GOL ×7

Waters ×193

PDB id:

4crw

Name:

Gene regulation

Title:

Complex of human ddx6 (reca-c) and cnot1 (mif4g)

Structure:

Ccr4-not transcription complex subunit 1. Chain: a. Fragment: cnot1 mif4g domain, residues 1093-1317. Synonym: cnot1, ccr4-associated factor 1, negative regulator of transcription subunit 1 homolog, not1h, hnot1. Engineered: yes. Probable atp-dependent RNA helicase ddx6. Chain: b. Fragment: ddx6 reca-c domain, residues 307-483.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: rosetta2.

Resolution:

1.75Å R-factor: 0.164 R-free: 0.203

Authors:

Y.Chen,A.Boland,E.Izaurralde,O.Weichenrieder

Key ref:

Y.Chen et al. (2014). A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct links between miRNA target recognition and silencing. Mol Cell, 54, 737-750. PubMed id: 24768540 DOI: 10.1016/j.molcel.2014.03.034

Date:

01-Mar-14 Release date: 07-May-14

Protein chain

A5YKK6  (CNOT1_HUMAN) -  CCR4-NOT transcription complex subunit 1 from Homo sapiens

Seq: 2376 a.a.

Struc: 226 a.a.*

Protein chain

P26196  (DDX6_HUMAN) -  Probable ATP-dependent RNA helicase DDX6 from Homo sapiens

Seq: 483 a.a.

Struc: 158 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain B: E.C.3.6.4.13 - Rna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1016/j.molcel.2014.03.034

Mol Cell 54:737-750 (2014)

PubMed id: 24768540

A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct links between miRNA target recognition and silencing.

Y.Chen, A.Boland, D.Kuzuoğlu-Öztürk, P.Bawankar, B.Loh, C.T.Chang, O.Weichenrieder, E.Izaurralde.

ABSTRACT

No abstract given.